质膜定位的钙离子泵和 Copines 协同调节拟南芥花粉萌发和育性。
Plasma Membrane-Localized Calcium Pumps and Copines Coordinately Regulate Pollen Germination and Fertility in Arabidopsis.
机构信息
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
Department of Anatomy, The Second Military Medical University, Shanghai 200433, China.
出版信息
Int J Mol Sci. 2018 Jun 15;19(6):1774. doi: 10.3390/ijms19061774.
Calcium plays an important role in plant growth, development, and response to environmental stimuli. Copines are conserved plasma membrane-localized calcium-binding proteins which regulate plant immune responses and development. In this study, we found that copine proteins BON2 and BON3, the paralogs of BON1, physically interact with calcium pumps ACA8 and ACA10 in Arabidopsis. Notably, ACA9, the closest homologue of ACA8 and ACA10 functioning in pollen tube growth, interacts with all three copines. This is consistent with the protein⁻protein interactions between the two protein families, the , , , mutants as well as mutant exhibited defects on pollen germination and seed production. Taken together, plasma membrane-localized interacting calcium pumps and copines coordinately control pollen tube growth, likely through manipulating calcium efflux.
钙在植物生长、发育和对环境刺激的反应中起着重要作用。Copines 是保守的质膜定位钙结合蛋白，调节植物免疫反应和发育。在这项研究中，我们发现 copine 蛋白 BON2 和 BON3，是 BON1 的同源物，与拟南芥中的钙泵 ACA8 和 ACA10 相互作用。值得注意的是，ACA9 是 ACA8 和 ACA10 的最接近同源物，在花粉管生长中起作用，与这三种 copines 都相互作用。这与这两个蛋白家族之间的蛋白-蛋白相互作用一致，即 、 、 、 突变体以及 突变体在花粉萌发和种子产生方面表现出缺陷。综上所述，质膜定位的相互作用钙泵和 copines 协同控制花粉管生长，可能通过操纵钙外排来实现。
Zotero 插件