Dipartimento di Biologia L. Gorini, Università degli Studi di Milano, Istituto di Biofisica del CNR, Sezione di Milano, Milano, Italy.
Plant Biol (Stuttg). 2011 May;13(3):421-30. doi: 10.1111/j.1438-8677.2010.00405.x. Epub 2010 Nov 3.
Ca(2+)-ATPases are P-type ATPases that use the energy of ATP hydrolysis to pump Ca(2+) from the cytoplasm into intracellular compartments or into the apoplast. Plant cells possess two types of Ca(2+) -pumping ATPase, named ECAs (for ER-type Ca(2+)-ATPase) and ACAs (for auto-inhibited Ca(2+)-ATPase). Each type comprises different isoforms, localised on different membranes. Here, we summarise available knowledge of the biochemical characteristics and the physiological role of plant Ca(2+)-ATPases, greatly improved after gene identification, which allows both biochemical analysis of single isoforms through heterologous expression in yeast and expression profiling and phenotypic analysis of single isoform knock-out mutants.
Ca(2+)-ATPases 是 P 型 ATPases，利用 ATP 水解的能量将 Ca(2+)从细胞质泵入细胞内隔室或质外体。植物细胞拥有两种类型的 Ca(2+)泵 ATPase，分别命名为 ECAs（内质网型 Ca(2+)-ATPase）和 ACAs（自动抑制型 Ca(2+)-ATPase）。每一种类型都包含不同的同工型，位于不同的膜上。在这里，我们总结了植物 Ca(2+)-ATPase 的生化特性和生理作用的现有知识，这些知识在基因鉴定后得到了极大的改善，这使得通过在酵母中异源表达对单个同工型进行生化分析，以及对单个同工型敲除突变体进行表达谱和表型分析成为可能。
