Heritage J, Bennett P M
J Gen Microbiol. 1985 May;131(5):1130-40. doi: 10.1099/00221287-131-5-1131.
We have observed plasmid fusions in a recA background mediated by a single end of TnA. These occur when transposase is provided either in cis or in trans. Insertions of the plasmid carrying the TnA inverted repeat sequence occur at many sites in the target plasmid. The point of fusion on the plasmid carrying TnA sequences always appears to be located in the region which carries the TnA inverted repeat sequence. In contrast to the transposition of an intact TnA element, plasmid fusions mediated by one end of TnA are very rare events. The implications of our results for models of transposition are discussed.
我们观察到在recA背景下由TnA的单一端介导的质粒融合。当转座酶以顺式或反式提供时，这些融合就会发生。携带TnA反向重复序列的质粒在靶质粒的许多位点发生插入。携带TnA序列的质粒上的融合点似乎总是位于携带TnA反向重复序列的区域。与完整TnA元件的转座不同，由TnA一端介导的质粒融合是非常罕见的事件。我们讨论了结果对转座模型的影响。
