对由Tn21单向转座产生的可变终点的分析。
Analysis of the variable endpoints generated by one-ended transposition of Tn21.
作者信息
Avila P, Grinsted J, de la Cruz F
机构信息
Departamento de Bioquímica, Facultad de Medicina, Polígono de Cazoña s/n, Santander, Spain.
出版信息
J Bacteriol. 1988 Mar;170(3):1350-3. doi: 10.1128/jb.170.3.1350-1353.1988.
One-ended transposition of Tn21 generates recombinants usually containing a whole copy of the donor replicon plus a short duplication of it (S. Mötsch, R. Schmitt, P. Avila, F. de la Crue, E. Ward, and J. Grinsted, Nucleic Acids Res. 13:3335-3342, 1985). This work shows that recombinants containing less than a whole copy of the donor replicon (hereafter called short recombinants) could also be detected when plasmid donors which contained two selectable genetic markers were used. Short recombinants were produced at the same frequency from TnpR+ donor molecules as from TnpR- donor molecules in a RecA- background. Therefore, they were not resolution products of larger recombinants. This result invalidates a previous hypothesis to explain one-ended transposition, that is, that one-ended transposition arises from the use of secondary ends by the transposition apparatus. On the other hand, it suggests that one-ended transposition of Tn21 occurs via a simple insertion mechanism.
Tn21 的单向转座通常会产生重组体，这些重组体通常包含供体复制子的完整拷贝以及它的一段短重复序列（S. 莫奇、R. 施密特、P. 阿维拉、F. 德拉克鲁、E. 沃德和 J. 格林斯特德，《核酸研究》13:3335 - 3342，1985 年）。这项研究表明，当使用含有两个可选择遗传标记的质粒供体时，也能够检测到包含少于完整供体复制子拷贝的重组体（以下称为短重组体）。在 RecA - 背景下，从 TnpR + 供体分子产生短重组体的频率与从 TnpR - 供体分子产生的频率相同。因此，它们不是较大重组体的解离产物。这一结果推翻了之前用于解释单向转座的一个假设，即单向转座源于转座装置对二级末端的利用。另一方面，这表明 Tn21 的单向转座是通过一种简单的插入机制发生的。
Zotero 插件