Roy P, Ritter G D, Akashi H, Collisson E, Inaba Y
J Gen Virol. 1985 Jul;66 ( Pt 7):1613-9. doi: 10.1099/0022-1317-66-7-1613.
We have used a DNA copy of segment 3 RNA of bluetongue virus serotype 17 (BTV-17) to detect sequence homology among the equivalent segments of five U.S.A. BTV serotypes (BTV-2, BTV-10, BTV-11, BTV-13 and BTV-17) as well as 14 other BTVs isolated from different endemic areas of the world. Both by in situ and Northern hybridization all the BTV serotypes were found to have RNA that reacted with the DNA probe. No homology was detected with epizootic haemorrhagic disease virus serotype 1, a related orbivirus. The BTV-17 DNA clone has also been used to detect viral RNA in infected sheep blood. This information has led us to develop a simple and sensitive procedure for the detection of viral genome-biotinylated clone DNA hybrids in vivo or in cultured cells following direct staining with either the avidin-fluorescein complex or the streptavidin-horseradish peroxidase complex.
我们已使用蓝舌病毒17型（BTV - 17）的第3节段RNA的DNA拷贝，来检测5种美国蓝舌病毒血清型（BTV - 2、BTV - 10、BTV - 11、BTV - 13和BTV - 17）以及从世界不同疫区分离出的其他14种蓝舌病毒的等效节段之间的序列同源性。通过原位杂交和Northern杂交发现，所有蓝舌病毒血清型都有能与该DNA探针发生反应的RNA。与相关的环状病毒—— epizootic haemorrhagic disease virus serotype 1未检测到同源性。BTV - 17 DNA克隆也已用于检测受感染绵羊血液中的病毒RNA。这些信息促使我们开发出一种简单而灵敏的方法，用于在体内或培养细胞中，在用抗生物素蛋白 - 荧光素复合物或链霉抗生物素蛋白 - 辣根过氧化物酶复合物直接染色后，检测病毒基因组 - 生物素化克隆DNA杂交体。
