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Detection of foot-and-mouth disease virus with DNA probes in bovine esophageal-pharyngeal fluids.

作者信息

Rossi M S, Sadir A M, Schudel A A, Palma E L

机构信息

Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias, Buenos Aires, Argentina.

出版信息

Arch Virol. 1988;99(1-2):67-74. doi: 10.1007/BF01311024.

DOI:10.1007/BF01311024
PMID:2833204
Abstract

Infectivity and dot-blot hybridization techniques were compared for the detection of FMDV in esophageal-pharyngeal fluids from experimentally infected cows. The probe used includes the viral polymerase sequence which allows the detection of the three types of virus (A, O, and C) with equivalent sensitivity. Virus was detected by dot-blot hybridization as well as by infectivity, according to sample analysis of esophageal-pharyngeal fluids extracted seven days post-infection. It was not possible to recover infective virus from some samples extracted at 180 and 560 days post-infection, although specific viral RNA was detected by dot-blot hybridization. This could indicate the presence of a high ratio of non-infective viral mutants in FMDV carrier cattle. These results emphasize the usefulness of molecular hybridization techniques for FMDV carrier-state detection.

摘要

相似文献

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引用本文的文献

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本文引用的文献

1
Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.动物微小核糖核酸病毒与植物豇豆花叶病毒在基因组织上的相似性以及蛋白质间的同源性表明，这些病毒家族有着共同的祖先。
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The complete nucleotide sequence of the RNA coding for the primary translation product of foot and mouth disease virus.口蹄疫病毒初级翻译产物编码RNA的完整核苷酸序列。
Nucleic Acids Res. 1984 Mar 12;12(5):2461-72. doi: 10.1093/nar/12.5.2461.
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Structure of the FMDV translation initiation site and of the structural proteins.
口蹄疫病毒持续存在的可能机制综述。
Epidemiol Infect. 1995 Feb;114(1):1-13. doi: 10.1017/s0950268800051864.
4
Applications of nucleic acid probes in veterinary infectious diseases.核酸探针在兽医传染病中的应用。
Vet Microbiol. 1990 Sep;24(3-4):409-17. doi: 10.1016/0378-1135(90)90187-z.
口蹄疫病毒翻译起始位点及结构蛋白的结构
Nucleic Acids Res. 1983 Nov 25;11(22):7873-85. doi: 10.1093/nar/11.22.7873.
4
Cloning of cDNA of major antigen of foot and mouth disease virus and expression in E. coli.口蹄疫病毒主要抗原的cDNA克隆及在大肠杆菌中的表达
Nature. 1981 Feb 12;289(5798):555-9. doi: 10.1038/289555a0.
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Hybridization of nucleic acids immobilized on solid supports.固定在固体支持物上的核酸杂交。
Anal Biochem. 1984 May 1;138(2):267-84. doi: 10.1016/0003-2697(84)90808-x.
6
A reproducible microanalytical method for the detection of specific RNA sequences by dot-blot hybridization.一种通过斑点杂交检测特定RNA序列的可重复微分析方法。
Anal Biochem. 1984 Feb;137(1):15-9. doi: 10.1016/0003-2697(84)90339-7.
7
Nucleotide sequence and genome organization of foot-and-mouth disease virus.口蹄疫病毒的核苷酸序列与基因组结构
Nucleic Acids Res. 1984 Aug 24;12(16):6587-601. doi: 10.1093/nar/12.16.6587.
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Further information on the persistence of infective foot-and-mouth disease virus in cattle exposed to virulent virus strains.
Bull Off Int Epizoot. 1966 Nov-Dec;65(11):1949-65.
9
A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6. doi: 10.1016/0006-291x(66)90447-5.
10
Studies on the carrier state of cattle exposed to foot-and-mouth disease virus.关于接触口蹄疫病毒的牛的带毒状态的研究。
J Hyg (Lond). 1966 Mar;64(1):81-90. doi: 10.1017/s0022172400040365.