脆弱拟杆菌临床分离株中隐蔽质粒的特征分析。

Characterisation of cryptic plasmids in clinical isolates of Bacteroides fragilis.

作者信息

Beul H A, von Eichel-Streiber C, Schreiner M, Schwindling F P, Weinblum D, Zöllner E J, Dierich M

出版信息

J Med Microbiol. 1985 Aug;20(1):39-48. doi: 10.1099/00222615-20-1-39.

Abstract

A screening method for plasmids in the fragilis group of Bacteroides spp. was developed, taking account of the lysozyme resistance of these species; 26 strains, 24 of them B. fragilis, were investigated by this method. Eleven strains contained plasmids and up to three different plasmids were found in individual strains. The plasmids belonged to five different size classes of mol. wt (10(6] 2.8, 3.5, 3.6, 4.2 and 19. Plasmids of equal size showed homology; no homology was found between plasmids of different sizes. Plasmids of equal size showed identical restriction patterns with 17 restriction endonucleases. Restriction maps were constructed for the five classes of plasmid.

摘要

考虑到脆弱拟杆菌属中这些菌种对溶菌酶的抗性，开发了一种用于筛选脆弱拟杆菌群中质粒的方法；用该方法对26株菌株进行了研究，其中24株为脆弱拟杆菌。11株菌株含有质粒，个别菌株中发现多达三种不同的质粒。这些质粒属于五个不同分子量大小类别（10⁶、2.8、3.5、3.将等大小的质粒显示出同源性；不同大小的质粒之间未发现同源性。等大小的质粒用17种限制性内切酶显示出相同的限制性图谱。构建了五类质粒的限制性图谱。6、4.2和19）。