Awad Romane A, Khalil Wagdy K B, Attallah Ashraf G
Department of Parasitology and Animal Diseases, Veterinary Division, National Research Center, 33 Bohouth St., 12622 Dokki, Giza, Egypt.
Department of Cell Biology, National Research Center, 33 Bohouth St., 12622 Dokki, Giza, Egypt.
Vet World. 2018 May;11(5):578-584. doi: 10.14202/vetworld.2018.578-584. Epub 2018 May 4.
This work aimed to study epidemiology and diagnosis of feline panleukopenia virus (FPV) using clinical examination, direct ELISA, RNA viral isolation and identification, and knowing phylogenetic tree of our isolate.
One hundred and sixty-five cats of different ages and sex were examined. Each cat was examined clinically to detect the clinical manifestations of the disease showing symptoms suggestive of feline panleukopenia (FP) as well as ELISA, and polymerase chain reaction (PCR) amplification analyses were conducted.
Our finding includes (a) clinical signs detected in 165 of 165 cats were in the form of lethargy, fever, anorexia, thirst, vomiting, diarrhea, dehydration, and leukopenia. (b) ELISA results revealed that 66 of all examined cats were positive for FPV. (c) The amplification products from all positive samples were confirmed as FPV (VP1) gene by nucleotide sequences analysis, in which 75 samples were positive using PCR amplification for the FPV. (d) Statistical evaluation of ELISA results in comparison to PCR findings. ELISA showed 88%, 100%, and 94.5% for sensitivity, specificity, and accuracy, respectively, while the prevalence of FP among the examined population was 45%. No effect of sex, breed, and age on ELISA results as recorded using Chi-square analysis.
The results of the sequence analysis indicated that PCR products of the FPV cDNA exhibited very low variation in their nucleotide sequence of all isolates compared with the published FPV genome, which could be suggested that FPV appears to be genomically stasis compared with other Parvoviruses. The genome sequence of FPLV strain in this study has been deposited in GenBank under the accession number KY466003. Our isolate closely related 100% to isolates from Portugal, which might be the origin of infection to Egypt through importation of cats.
本研究旨在通过临床检查、直接酶联免疫吸附测定（ELISA）、RNA病毒分离与鉴定以及了解我们分离株的系统发育树，来研究猫泛白细胞减少症病毒（FPV）的流行病学和诊断方法。
对165只不同年龄和性别的猫进行了检查。对每只猫进行临床检查，以检测出表现出猫泛白细胞减少症（FP）症状的疾病临床表现，并进行ELISA以及聚合酶链反应（PCR）扩增分析。
我们的研究结果包括：（a）165只猫中检测到的临床症状表现为嗜睡、发热、厌食、口渴、呕吐、腹泻、脱水和白细胞减少。（b）ELISA结果显示，所有检查的猫中有66只为FPV阳性。（c）通过核苷酸序列分析，所有阳性样本的扩增产物被确认为FPV（VP1）基因，其中75个样本通过PCR扩增检测为FPV阳性。（d）ELISA结果与PCR结果的统计学评估。ELISA的灵敏度、特异性和准确性分别为88%、100%和94.5%，而在所检查的猫群中FP的患病率为45%。使用卡方分析记录的性别、品种和年龄对ELISA结果均无影响。
序列分析结果表明，与已发表的FPV基因组相比，FPV cDNA的PCR产物在所有分离株的核苷酸序列中表现出非常低的变异，这可能表明与其他细小病毒相比，FPV在基因组上似乎处于停滞状态。本研究中FPLV毒株的基因组序列已保存在GenBank中，登录号为KY466003。我们的分离株与来自葡萄牙的分离株100%密切相关，这可能是通过进口猫传入埃及的感染源。
