Decaro N, Desario C, Miccolupo A, Campolo M, Parisi A, Martella V, Amorisco F, Lucente M S, Lavazza A, Buonavoglia C
Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine, Strada per Casamassima km 3, 70010 Valenzano (BA), Italy.
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, via Manfredonia 20, 71100 Foggia, Italy.
J Gen Virol. 2008 Sep;89(Pt 9):2290-2298. doi: 10.1099/vir.0.2008/001503-0.
Thirty-nine parvovirus strains contained in faecal samples collected in Italy (n=34) and UK (n=5) from cats with feline panleukopenia were characterized at the molecular level. All viruses were proven to be true feline panleukopenia virus (FPLV) strains by a minor groove binder probe assay, which is able to discriminate between FPLV and the closely related canine parvovirus type 2. By using sequence analysis of the VP2 gene, it was found that the FPLV strains detected in Italy and UK were highly related to each other, with a nucleotide identity of 99.1-100 and 99.4-99.8% among Italian and British strains, respectively, whereas the similarities between all the sequences analysed were 98.6-100%. Eighty-eight variable positions were detected in the VP2 gene of the field and reference FPLV strains, most of which were singletons. Synonymous substitutions (n=57) predominated over non-synonymous substitutions (n=31), and the ratio between synonymous and non-synonymous substitutions (dN/dS) was 0.10, thus confirming that evolution of FPLV is driven by random genetic drift rather than by positive selection pressure. Some amino acid mutations in the VP2 protein affected sites that are thought to be responsible for antigenic and biological properties of the virus, but no clear patterns of segregation and genetic markers, were identified, confirming that FPLV is in evolutionary stasis.
对从意大利（n = 34）和英国（n = 5）收集的患有猫泛白细胞减少症的猫的粪便样本中所含的39株细小病毒进行了分子水平的特征分析。通过小沟结合剂探针检测，所有病毒均被证实为真正的猫泛白细胞减少症病毒（FPLV）毒株，该检测能够区分FPLV和密切相关的犬细小病毒2型。通过对VP2基因进行序列分析，发现意大利和英国检测到的FPLV毒株彼此高度相关，意大利毒株和英国毒株之间的核苷酸同一性分别为99.1 - 100%和99.4 - 99.8%，而所有分析序列之间的相似性为98.6 - 100%。在野外和参考FPLV毒株的VP2基因中检测到88个可变位点,其中大多数是单态性的。同义替换（n = 57）多于非同义替换（n = 31），同义替换与非同义替换的比率（dN/dS）为0.10，从而证实FPLV的进化是由随机遗传漂变而非正选择压力驱动的。VP2蛋白中的一些氨基酸突变影响了被认为与病毒抗原性和生物学特性有关的位点，但未发现明显的分离模式和遗传标记，这证实FPLV处于进化停滞状态。
