• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

巴雷特食管上皮中转化生长因子β的低表达

Low Expression of Transforming Growth Factor β in the Epithelium of Barrett's Esophagus.

作者信息

Lee Shou-Wu, Lien Han-Chung, Lin Chi-Chen, Wen Mei-Chin, Chang Chi-Sen

机构信息

Division of Gastroenterology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.

Department of Internal Medicine, Chung Shan Medical University, Taichung, Taiwan.

出版信息

Gastroenterology Res. 2018 Jun;11(3):189-194. doi: 10.14740/gr1009w. Epub 2018 May 31.

DOI:10.14740/gr1009w
PMID:29915628
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5997480/
Abstract

BACKGROUND

The aim of this study was to investigate the expression of transforming growth factor β (TGF-β) in the different stages of Barrett's esophagus (BE).

METHODS

Paired endoscopic esophageal biopsy samples were obtained from patients with BE prospectively. Subjects were classified into three groups: BE, BE with dysplasia, and adenocarcinoma (AC) arising from BE. Biopsy specimens over normal esophageal epithelium and gastric cardiac epithelium of limited cases were done. Four cell lines, HETA1 (human esophageal epithelium), CA-A and CP-C (non-dysplastic metaplasia), and OE33 (AC) were analyzed for quantitative mRNA and Western blotting of TGF-β.

RESULTS

All 30 subjects with BE were enrolled. Expression of TGF-β mRNA in BE were significantly (P < 0.01) lower than that in the normal esophagus and cardiac epithelium. The BE tissue showed a lower positive ratio of TGF-β immunohistochemical (IHC) stain than the cardiac epithelium. The expression of TGF-β mRNA in the cell lines CA-A, CP-3, OE-33, was significantly (P < 0.05) lower than that in the cell line HETA-1. The Western blotting result showed lower TGF-β protein expression of the cell lines CA-A, CP-3, and OE-33.

CONCLUSIONS

The expression of TGF-β was lower in the tissue of BE.

摘要

背景

本研究旨在调查转化生长因子β（TGF-β）在巴雷特食管（BE）不同阶段的表达情况。

方法

前瞻性地从BE患者中获取配对的内镜食管活检样本。受试者分为三组：BE、伴有发育异常的BE和由BE引发的腺癌（AC）。对有限病例的正常食管上皮和胃贲门上皮进行活检标本检测。分析四种细胞系，即HETA1（人食管上皮）、CA-A和CP-C（非发育异常化生）以及OE33（AC）中TGF-β的定量mRNA和蛋白质印迹。

结果

共纳入30例BE受试者。BE中TGF-β mRNA的表达显著低于正常食管和贲门上皮（P < 0.01）。BE组织中TGF-β免疫组化（IHC）染色的阳性率低于贲门上皮。细胞系CA-A、CP-3、OE-33中TGF-β mRNA的表达显著低于细胞系HETA-1（P < 0.05）。蛋白质印迹结果显示细胞系CA-A、CP-3和OE-33中TGF-β蛋白表达较低。

结论

BE组织中TGF-β的表达较低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/66daa3081dec/gr-11-189-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/b6262c4eeef6/gr-11-189-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/551ff01f9f51/gr-11-189-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/437f71783056/gr-11-189-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/b62172543856/gr-11-189-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/66daa3081dec/gr-11-189-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/b6262c4eeef6/gr-11-189-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/551ff01f9f51/gr-11-189-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/437f71783056/gr-11-189-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/b62172543856/gr-11-189-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/840f/5997480/66daa3081dec/gr-11-189-g005.jpg

相似文献

1
Low Expression of Transforming Growth Factor β in the Epithelium of Barrett's Esophagus.巴雷特食管上皮中转化生长因子β的低表达
Gastroenterology Res. 2018 Jun;11(3):189-194. doi: 10.14740/gr1009w. Epub 2018 May 31.
2
In-depth characterization of the Wnt-signaling/β-catenin pathway in an in vitro model of Barrett's sequence.在巴雷特食管序列的体外模型中对Wnt信号/β-连环蛋白通路进行深入表征。
BMC Gastroenterol. 2019 Mar 6;19(1):38. doi: 10.1186/s12876-019-0957-5.
3
ErbB2 without erbB3 expression in metaplastic columnar epithelium of Barrett's esophagus.在巴雷特食管化生柱状上皮中无erbB3表达的ErbB2
Digestion. 2004;70(2):95-102. doi: 10.1159/000080928. Epub 2004 Sep 16.
4
Transforming growth factor-alpha, epidermal growth factor receptor, and MiB-1 expression in Barrett's-associated neoplasia: correlation with prognosis.转化生长因子-α、表皮生长因子受体及MiB-1在巴雷特相关肿瘤中的表达：与预后的相关性
Mod Pathol. 1997 Feb;10(2):105-12.
5
Proinflammatory cytokine and nuclear factor kappa-B expression along the inflammation-metaplasia-dysplasia-adenocarcinoma sequence in the esophagus.食管中炎症-化生-发育异常-腺癌序列中促炎细胞因子和核因子κB的表达
Am J Gastroenterol. 2005 Jun;100(6):1257-64. doi: 10.1111/j.1572-0241.2005.41338.x.
6
Expression of the putative stem cell marker Musashi-1 in Barrett's esophagus and esophageal adenocarcinoma.Musashi-1 作为一种假定的干细胞标志物在 Barrett 食管和食管腺癌中的表达。
Dis Esophagus. 2010 Sep;23(7):580-9. doi: 10.1111/j.1442-2050.2010.01061.x. Epub 2010 Apr 29.
7
Endoscopic features of buried Barrett's mucosa.内镜下埋置型 Barrett 黏膜的特征。
Gastrointest Endosc. 2021 Jul;94(1):14-21. doi: 10.1016/j.gie.2020.12.031. Epub 2020 Dec 26.
8
Usefulness of insulin-like growth factor II mRNA-binding protein 3 (IMP3) as a new marker for the diagnosis of esophageal adenocarcinoma in challenging cases.胰岛素样生长因子II mRNA结合蛋白3（IMP3）作为诊断疑难病例食管腺癌新标志物的效用。
Turk J Gastroenterol. 2014 Jun;25(3):253-6. doi: 10.5152/tjg.2014.5454.
9
Aberrant epithelial-mesenchymal Hedgehog signaling characterizes Barrett's metaplasia.异常的上皮-间充质 Hedgehog 信号特征表现为巴雷特食管化生。
Gastroenterology. 2010 May;138(5):1810-22. doi: 10.1053/j.gastro.2010.01.048. Epub 2010 Feb 4.
10
Increased CDX2 and decreased PITX1 homeobox gene expression in Barrett's esophagus and Barrett's-associated adenocarcinoma.巴雷特食管及巴雷特相关腺癌中CDX2表达增加而PITX1同源框基因表达减少。
Surgery. 2005 Nov;138(5):924-31. doi: 10.1016/j.surg.2005.05.007.

引用本文的文献

1
Global Transcriptomic Analysis of Topical Sodium Alginate Protection against Peptic Damage in an In Vitro Model of Treatment-Resistant Gastroesophageal Reflux Disease.局部应用海藻酸钠预防难治性胃食管反流病的体外模型中消化性损伤的全球转录组分析。
Int J Mol Sci. 2024 Oct 5;25(19):10714. doi: 10.3390/ijms251910714.
2
TGF-β Signaling in Liver, Pancreas, and Gastrointestinal Diseases and Cancer.TGF-β 信号在肝脏、胰腺和胃肠道疾病及癌症中的作用。
Gastroenterology. 2021 Aug;161(2):434-452.e15. doi: 10.1053/j.gastro.2021.04.064. Epub 2021 Apr 30.
3
Pathologically Complete Response after Triple Therapy in Locally Advanced Esophageal Cancer in a Hereditary Hemorrhagic Telangiectasia Patient.

本文引用的文献

1
Quality indicators for the management of Barrett's esophagus, dysplasia, and esophageal adenocarcinoma: international consensus recommendations from the American Gastroenterological Association Symposium.巴雷特食管、发育异常和食管腺癌管理的质量指标：美国胃肠病学会研讨会的国际共识建议
Gastroenterology. 2015 Nov;149(6):1599-606. doi: 10.1053/j.gastro.2015.08.007. Epub 2015 Aug 19.
2
TGFβ1 and HGF protein secretion by esophageal squamous epithelial cells and stromal fibroblasts in oesophageal carcinogenesis.食管癌变过程中食管鳞状上皮细胞和基质成纤维细胞分泌转化生长因子β1和肝细胞生长因子蛋白的情况。
Oncol Lett. 2013 Aug;6(2):401-406. doi: 10.3892/ol.2013.1409. Epub 2013 Jun 18.
3
遗传性出血性毛细血管扩张症患者局部晚期食管癌三联疗法后的病理完全缓解
Case Rep Oncol. 2020 Feb 18;13(1):176-181. doi: 10.1159/000505832. eCollection 2020 Jan-Apr.
4
From genetics to signaling pathways: molecular pathogenesis of esophageal adenocarcinoma.从遗传学到信号通路：食管腺癌的分子发病机制。
Biochim Biophys Acta Rev Cancer. 2019 Aug;1872(1):37-48. doi: 10.1016/j.bbcan.2019.05.003. Epub 2019 May 30.
Loss of TGF-β adaptor β2SP activates notch signaling and SOX9 expression in esophageal adenocarcinoma.
TGF-β 接头蛋白 β2SP 的缺失激活食管腺癌中的 Notch 信号通路和 SOX9 表达。
Cancer Res. 2013 Apr 1;73(7):2159-69. doi: 10.1158/0008-5472.CAN-12-1962. Epub 2013 Mar 27.
4
Barrett's metaplasia as a paradigm for understanding the development of cancer.巴雷特食管化生作为理解癌症发展的范例。
Curr Opin Genet Dev. 2012 Oct;22(5):494-9. doi: 10.1016/j.gde.2012.08.001. Epub 2012 Sep 11.
5
Clinical practice. Barrett's esophagus.临床实践。巴雷特食管。
N Engl J Med. 2009 Dec 24;361(26):2548-56. doi: 10.1056/NEJMcp0902173.
6
TGFbeta in Cancer.癌症中的转化生长因子β
Cell. 2008 Jul 25;134(2):215-30. doi: 10.1016/j.cell.2008.07.001.
7
Selective loss of TGFbeta Smad-dependent signalling prevents cell cycle arrest and promotes invasion in oesophageal adenocarcinoma cell lines.选择性丧失 TGFβ Smad 依赖性信号转导可防止食管腺癌细胞系的细胞周期停滞并促进侵袭。
PLoS One. 2007 Jan 31;2(1):e177. doi: 10.1371/journal.pone.0000177.
8
The development and validation of an endoscopic grading system for Barrett's esophagus: the Prague C & M criteria.巴雷特食管内镜分级系统的开发与验证：布拉格C&M标准
Gastroenterology. 2006 Nov;131(5):1392-9. doi: 10.1053/j.gastro.2006.08.032. Epub 2006 Aug 16.
9
The Montreal definition and classification of gastroesophageal reflux disease: a global evidence-based consensus.《蒙特利尔胃食管反流病的定义与分类：一项全球循证共识》
Am J Gastroenterol. 2006 Aug;101(8):1900-20; quiz 1943. doi: 10.1111/j.1572-0241.2006.00630.x.
10
Impaired transforming growth factor beta signalling in Barrett's carcinogenesis due to frequent SMAD4 inactivation.由于SMAD4频繁失活，巴雷特癌变过程中转化生长因子β信号传导受损。
Gut. 2006 Jun;55(6):764-74. doi: 10.1136/gut.2005.076430. Epub 2005 Dec 20.