O'Donnell P V, Fleissner E, Lonial H, Koehne C F, Reicin A
J Virol. 1985 Aug;55(2):500-3. doi: 10.1128/JVI.55.2.500-503.1985.
Blot hybridization of thymocyte DNA from AKR/J mice was used to detect new proviral junction fragments as markers of clonality at different stages of viral leukemogenesis and to detect DNA rearrangements at the c-myc locus due to proviral insertion. Clonal populations of thymocytes were observed in mink cell focus-forming virus-injected mice as early as 35 days postinjection, at a stage distinguishable from frank leukemia by flow cytometric analysis and transplantation bioassay. Specific proviral integrations in the c-myc locus were detected in 15% of these early clones and in up to 65% of late-developing thymomas and frank leukemias. Thus, in this system c-myc activation appears to be a common mechanism in T-cell leukemogenesis.
利用来自AKR/J小鼠的胸腺细胞DNA进行印迹杂交，以检测新的前病毒连接片段，作为病毒白血病发生不同阶段克隆性的标志物，并检测由于前病毒插入导致的c-myc基因座处的DNA重排。早在注射貂细胞集落形成病毒后的35天，在注射小鼠中就观察到了胸腺细胞的克隆群体，此时通过流式细胞术分析和移植生物测定法可将其与明显的白血病区分开来。在这些早期克隆的15%以及后期发展的胸腺瘤和明显白血病的65%中检测到了c-myc基因座中的特异性前病毒整合。因此，在该系统中，c-myc激活似乎是T细胞白血病发生的常见机制。
