Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China.
Medical Experiment Center, Shaanxi University of Chinese Medicine, Xianyang, 712046, Shaanxi, People's Republic of China.
Biol Trace Elem Res. 2019 Feb;187(2):499-505. doi: 10.1007/s12011-018-1405-z. Epub 2018 Jun 19.
Fluoride had been reported to damage the structure and function of testicular tissues and reproductive cells; however, the mechanisms underlying its toxicity remained unclear. Autophagy plays a key role in reproductive function. In this study, we aimed to investigate the effect of fluoride on autophagy in Sertoli cells. Sertoli cells were exposed to 0, 0.125, 0.25, and 0.5 mM NaF for 24 h. The results showed that fluoride exposure up-regulated Beclin1 and p62 mRNA and protein expression levels with concomitant down-regulated mRNA and protein expression levels of LC3 and Atg5. In conclusion, exposure to fluoride impaired the autophagy process in Sertoli cells, which could be one of fluoride's mechanisms in male reproductive toxicity.
氟化物已被报道会损害睾丸组织和生殖细胞的结构和功能；然而，其毒性的机制尚不清楚。自噬在生殖功能中起着关键作用。在这项研究中，我们旨在研究氟化物对支持细胞自噬的影响。将支持细胞暴露于 0、0.125、0.25 和 0.5 mM NaF 中 24 小时。结果表明，氟化物暴露会上调 Beclin1 和 p62 mRNA 和蛋白表达水平，同时下调 LC3 和 Atg5 的 mRNA 和蛋白表达水平。总之，暴露于氟化物会损害支持细胞中的自噬过程，这可能是氟化物引起男性生殖毒性的机制之一。
