Smiley M L, Friedman H M
J Virol. 1985 Sep;55(3):857-61. doi: 10.1128/JVI.55.3.857-861.1985.
Neuraminidase treatment of cells infected with herpes simplex virus type 1 (HSV-1) markedly enhanced the binding of complement component C3b to HSV 1 glycoprotein C (gC). When HSV-1 was grown in BHK RicR14 cells in which glycoproteins had reduced amounts of N-linked complex oligosaccharides, including sialic acid, the binding of C3b to gC was markedly enhanced. We used neuraminidase treatment to demonstrate that cloning the gC gene from the HSV-1 F strain into an HSV-1 mutant which fails to express gC converted the mutant virus from C3b receptor negative to receptor positive. These results further support a role for gC as a C3b receptor and indicate that sialic acid modifies receptor activity.
用神经氨酸酶处理感染单纯疱疹病毒1型（HSV-1）的细胞，可显著增强补体成分C3b与HSV 1糖蛋白C（gC）的结合。当HSV-1在BHK RicR14细胞中生长时，其中糖蛋白的N-连接复合寡糖（包括唾液酸）含量减少，C3b与gC的结合显著增强。我们用神经氨酸酶处理证明，将HSV-1 F株的gC基因克隆到一个不表达gC的HSV-1突变体中，可使突变病毒从C3b受体阴性转变为受体阳性。这些结果进一步支持了gC作为C3b受体的作用，并表明唾液酸可修饰受体活性。
