Rojo-Laguna José I, Saló Emili
Department of Genetics Microbiology and Statistics and Institute of Biomedicine IBUB, University of Barcelona, Barcelona, Catalonia, Spain.
Methods Mol Biol. 2018;1774:497-505. doi: 10.1007/978-1-4939-7802-1_21.
Tissue transplantation is an important tool for in vivo studies of pattern organization and axis establishment or maintenance in planarians. Further, transplants can inform on cell movements under different regenerative conditions or genetic backgrounds. Here, we detail classical grafting strategies that have been variously employed in planarian research over the last few decades. These protocols remain similarly useful for addressing current and future questions in the field and the step-by-step protocol that we provide allows facile adaptation of this important method into the experimental repertoire of a laboratory.
组织移植是用于涡虫体内模式组织、轴建立或维持研究的重要工具。此外，移植可以揭示不同再生条件或遗传背景下的细胞运动情况。在这里，我们详细介绍了过去几十年在涡虫研究中广泛应用的经典移植策略。这些方案对于解决该领域当前和未来的问题仍然同样有用，并且我们提供的分步方案能够让这个重要方法轻松融入实验室的实验方法库中。
