a The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.
b Women&infants Hospital Of Zhengzhou , Zhengzhou , China.
Artif Cells Nanomed Biotechnol. 2018;46(sup2):1120-1126. doi: 10.1080/21691401.2018.1479711. Epub 2018 Jun 19.
The human cervical carcinoma oncogenic mechanisms still remain elusive. Thus, we proposed to understand the biological role of a newly discovered therapeutic miRNA.
MiR-1297 related to human cervical carcinoma was selected for this study. TaqMan qRT- PCR assay was used to profile miRNA, phosphatase and tensin homolog (PTEN) expression in randomly chosen tumour with non-tumour tissues, and the apoptosis factors expression. Cell proliferation was monitored by CCK-8 assay and colony formation assay. Apoptosis was determined by flow cytometry. Protein level was determined by western blotting. 3'UTR was performed to validate the direct binding sites of miR-1297 on PTEN. SPSS was used for statistical analyses.
MiR-1297 is repressed and PTEN activated in human cervical cancer tissues. After miR-1297 overexpression, HeLa cells had an increase in cell proliferation and decrease in apoptosis. PTEN expression is negatively correlation with miR-1297. PTEN silencing display the similar pattern as miRNA-1297 overexpression to inhibit HeLa cell growth and apoptosis in vitro.
Our data indicate that miR-1297 contribute to the human cervical carcinoma through PTEN. miR-1297 could be a reasonable miRNA for future studies.
人类宫颈癌的致癌机制仍不清楚。因此，我们拟研究一个新发现的治疗性 miRNA 的生物学作用。
选择与宫颈癌相关的 miR-1297。TaqMan qRT-PCR 法检测随机选择的肿瘤组织和非肿瘤组织中 miRNA、磷酸酶和张力蛋白同源物（PTEN）的表达，并检测凋亡因子的表达。通过 CCK-8 法和集落形成实验监测细胞增殖。通过流式细胞术测定细胞凋亡。通过 Western blot 法测定蛋白水平。采用 3'UTR 验证 miR-1297 对 PTEN 的直接结合位点。采用 SPSS 进行统计学分析。
miR-1297 在宫颈癌组织中受抑制，PTEN 被激活。过表达 miR-1297 后，HeLa 细胞的增殖增加，凋亡减少。PTEN 表达与 miR-1297 呈负相关。沉默 PTEN 可抑制 HeLa 细胞体外生长和凋亡，与 miR-1297 过表达的表型相似。
我们的数据表明，miR-1297 通过 PTEN 促进了人类宫颈癌的发生。miR-1297 可能是未来研究的合理 miRNA。
