Department of Obstetrics and Gynecology, The First Affiliated Hospital of Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia, PR China.
Department of Respiratory, The Third Affiliated Hospital of Inner Mongolia Medical College, Baotou, PR China.
Kaohsiung J Med Sci. 2021 Nov;37(11):964-972. doi: 10.1002/kjm2.12443. Epub 2021 Sep 25.
Circular RNAs (circRNAs) play prominent roles in regulating the progression of cancers. This study is aimed to decipher the role of hsa_circ_0000730 in cervical cancer (CC).The differentially expressed circRNAs of CC were screened out from the Gene Expression Omnibus database. qRT-PCR was used to detect circ_0000730 expression in CC tissues and cell lines, and the Kaplan-Meier curve was adopted to figure out the relationship between circ_000730 expression and the overall survival time of CC patients. BrdU assay and Tanswell assay were utilized to examine the proliferation, migration, and invasion of CC cells. Western blot was adopted to detect PTEN protein expression. Bioinformatics analysis and dual-luciferase reporter assay were used to examine the target relationship between miR-942-5p and circ_0000730 or PTEN, respectively.Circ_0000730 was among the differentially expressed circRNAs in CC. Circ_0000730 was significantly down-regulated in the cancer tissues of 50 CC patients and CC cell lines. Additionally, underexpression of circ_0000730 was associated with the shorter survival time of CC patients. Gain- and loss-of-function assays highlighted that circ_0000730 significantly inhibited the proliferation, migration, and invasion of CC cells. Mechanistically, miR-942-5p was identified as a downstream target of circ_0000730, and circ_0000730 could positively regulate PTEN expression via repressing miR-942-5p in CC cells.Circ_0000730 inhibits the proliferation, migration, and invasion of CC cells via regulating miR-942-5p/PTEN axis. Circ_0000730 probably acts as a tumor suppressor in CC, and it may be a candidate target for the treatment of CC.
环状 RNA（circRNAs）在调控癌症进展中发挥重要作用。本研究旨在解析 hsa_circ_0000730 在宫颈癌（CC）中的作用。从基因表达综合数据库筛选出 CC 差异表达的 circRNAs。qRT-PCR 检测 CC 组织和细胞系中 circ_0000730 的表达，采用 Kaplan-Meier 曲线分析 circ_000730 表达与 CC 患者总生存时间的关系。BrdU 检测和 Transwell 检测用于检测 CC 细胞的增殖、迁移和侵袭。Western blot 检测 PTEN 蛋白表达。生物信息学分析和双荧光素酶报告实验分别用于检测 miR-942-5p 与 circ_0000730 或 PTEN 的靶向关系。Circ_0000730 是 CC 中差异表达的 circRNAs 之一。50 例 CC 患者和 CC 细胞系的癌组织中 circ_0000730 显著下调。此外，circ_0000730 低表达与 CC 患者的生存时间较短有关。增益和缺失功能实验强调，circ_0000730 显著抑制 CC 细胞的增殖、迁移和侵袭。机制上，miR-942-5p 被鉴定为 circ_0000730 的下游靶标，circ_0000730 可通过抑制 CC 细胞中 miR-942-5p 来正向调节 PTEN 表达。Circ_0000730 通过调节 miR-942-5p/PTEN 轴抑制 CC 细胞的增殖、迁移和侵袭。Circ_0000730 在 CC 中可能作为肿瘤抑制因子发挥作用，它可能是 CC 治疗的候选靶点。
