Yasuda H, Okazaki T
Nucleic Acids Res. 1985 Jul 11;13(13):4953-69. doi: 10.1093/nar/13.13.4953.
Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to triribonucleotide stretches were isolated at the 5' end of the short DNA chains. A structure for the novel oligonucleotide is proposed to be p5' X3' pp5' rN, where X represents unidentified nucleoside with a peculiar property. Possible metabolic relationship between these molecules and primer RNA has been discussed.
与枯草芽孢杆菌短DNA链5'末端共价连接的碱不稳定部分，即推测的用于不连续DNA合成的引物RNA，使用温度敏感的DNA聚合酶I突变体进行分离和分析，该突变体在限制温度下积累新生DNA片段。在短DNA链的5'末端分离出一种新型寡核苷酸结构以及单至三核糖核苷酸片段。提出该新型寡核苷酸的结构为p5' X3' pp5' rN，其中X代表具有特殊性质的未鉴定核苷。已讨论了这些分子与引物RNA之间可能的代谢关系。
