Christophe D, Cabrer B, Bacolla A, Targovnik H, Pohl V, Vassart G
Nucleic Acids Res. 1985 Jul 25;13(14):5127-44. doi: 10.1093/nar/13.14.5127.
A region of human genomic DNA encompassing the 5' end of the thyroglobulin gene has been sequenced and the position of the transcriptional start site has been determined. The 5' non-translated portion of the mRNA displays a quasi-palindromic sequence which could allow this region to adopt a hairpin structure. The first exon of the gene encodes a 19 amino-acids signal peptide and the 3 first amino acids of the mature protein. Apart from the canonical TATA-Box and from a CAAT-Box homology, the promoter region contains a 209 bp-long poly(purine)-poly (pyrimidine) sequence located between positions-512 and -304 relative to the transcription start. When contained in a supercoiled plasmid, this sequence exhibits sensitivity to S1 nuclease at two distinct positions. A precise mapping of the borders of the sensitive regions was achieved by extending primers from both ends of the sequence after digestion by the enzyme. The resulting data can be explained by a model involving the formation of a triple helix structure.
对包含甲状腺球蛋白基因5'端的一段人类基因组DNA区域进行了测序，并确定了转录起始位点的位置。mRNA的5'非翻译部分呈现出准回文序列，这可能使该区域形成发夹结构。该基因的第一个外显子编码一个19个氨基酸的信号肽和成熟蛋白的前3个氨基酸。除了典型的TATA盒和CAAT盒同源序列外，启动子区域还包含一个位于相对于转录起始点-512至-304位置之间的209 bp长的聚嘌呤-聚嘧啶序列。当包含在超螺旋质粒中时，该序列在两个不同位置对S1核酸酶敏感。通过在酶消化后从序列两端延伸引物，实现了对敏感区域边界的精确映射。所得数据可以用一个涉及三螺旋结构形成的模型来解释。
