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表皮生长因子受体基因的嘧啶/嘌呤偏向区对S1核酸酶敏感，可能形成分子内三链体。

The pyrimidine/purine-biased region of the epidermal growth factor receptor gene is sensitive to S1 nuclease and may form an intramolecular triplex.

作者信息

Kato M, Kudoh J, Shimizu N

机构信息

Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.

出版信息

Biochem J. 1990 May 15;268(1):175-80. doi: 10.1042/bj2680175.

DOI:10.1042/bj2680175
PMID:2344356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1131408/
Abstract

The pyrimidine/purine-biased region located upstream of the EGF (epidermal growth factor) receptor gene transcription initiation sites was sensitive to S1 nuclease when under superhelical tension. The structural basis of this specific reactivity to S1 nuclease was probed by the use of diethyl pyrocarbonate. The patterns of modification suggested that the H-form proposed by Mirkin, Lyamichev, Drushlyak, Dobrynin, Filippov & Frank-Kamenetskii [Nature (London) (1987) 330, 495-497], which includes an intramolecular triplex and a single-stranded region, was the most plausible model for the sequence tested. The results of dimethyl sulphate modification also supported this model.

摘要

位于表皮生长因子（EGF）受体基因转录起始位点上游的嘧啶/嘌呤偏倚区在超螺旋张力下对S1核酸酶敏感。通过使用焦碳酸二乙酯探究了对S1核酸酶这种特异性反应性的结构基础。修饰模式表明，米尔金、利亚米切夫、德鲁什利亚克、多布林宁、菲利波夫和弗兰克-卡梅涅茨基[《自然》（伦敦）（1987年）330卷，495 - 497页]提出的H型结构，包括一个分子内三链体和一个单链区，是所测试序列最合理的模型。硫酸二甲酯修饰的结果也支持该模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/f558328aedbf/biochemj00183-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/3e02cb56e579/biochemj00183-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/15efc8c0b3be/biochemj00183-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/f558328aedbf/biochemj00183-0174-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/3e02cb56e579/biochemj00183-0173-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/15efc8c0b3be/biochemj00183-0174-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed36/1131408/f558328aedbf/biochemj00183-0174-b.jpg

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1
The pyrimidine/purine-biased region of the epidermal growth factor receptor gene is sensitive to S1 nuclease and may form an intramolecular triplex.表皮生长因子受体基因的嘧啶/嘌呤偏向区对S1核酸酶敏感，可能形成分子内三链体。
Biochem J. 1990 May 15;268(1):175-80. doi: 10.1042/bj2680175.
2
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引用本文的文献

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Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIPs.使用寡聚三链体通过混合嘌呤/嘧啶DNA序列形成三链体进行分子识别。
J Am Chem Soc. 2005 Sep 14;127(36):12657-65. doi: 10.1021/ja0530218.
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Chromatin structure of the EGFR gene suggests a role for intron 1 sequences in its regulation in breast cancer cells.表皮生长因子受体（EGFR）基因的染色质结构表明，内含子1序列在乳腺癌细胞中对该基因的调控发挥作用。
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3
Polypyrimidine/polypurine sequence in plasmid DNA enhances formation of dimer molecules in Escherichia coli. Dimerization of plasmid DNA in Escherichia coli.

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An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.通过S1核酸酶检测到的DNA构象改变发生在活跃的鸡珠蛋白染色质的特定区域。
Cell. 1982 Jun;29(2):609-22. doi: 10.1016/0092-8674(82)90177-5.
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