Gelman I H, Silverstein S
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5265-9. doi: 10.1073/pnas.82.16.5265.
A HeLa cell transient-expression assay system was used to determine if isolated immediate early (alpha) genes from herpes simplex virus (HSV) could transcriptionally activate (transactivate) the type 1 (HSV-1) thymidine kinase (TK) gene [an early (beta) gene]. Cells transfected with the TK gene alone transcribed very low levels of TK RNA. Cells cotransfected with plasmids bearing the sequences that encode the alpha-gene product infected cell protein 0 or 4 (ICP0 or ICP4) and the TK gene faithfully transcribed high levels of TK RNA. The plasmid containing the sequences encoding ICP0 was a more potent transactivator than the plasmid containing the sequences for ICP4.
使用HeLa细胞瞬时表达检测系统来确定从单纯疱疹病毒（HSV）中分离出的立即早期（α）基因是否能够转录激活1型（HSV-1）胸苷激酶（TK）基因[一种早期（β）基因]。单独转染TK基因的细胞转录出的TK RNA水平非常低。与携带编码α基因产物感染细胞蛋白0或4（ICP0或ICP4）序列的质粒和TK基因共转染的细胞忠实地转录出高水平的TK RNA。含有编码ICP0序列的质粒比含有ICP4序列的质粒是更有效的反式激活因子。
