从单纯疱疹病毒中鉴定出可反式激活病毒胸苷激酶基因的立即早期基因。
Identification of immediate early genes from herpes simplex virus that transactivate the virus thymidine kinase gene.
作者信息
Gelman I H, Silverstein S
出版信息
Proc Natl Acad Sci U S A. 1985 Aug;82(16):5265-9. doi: 10.1073/pnas.82.16.5265.
A HeLa cell transient-expression assay system was used to determine if isolated immediate early (alpha) genes from herpes simplex virus (HSV) could transcriptionally activate (transactivate) the type 1 (HSV-1) thymidine kinase (TK) gene [an early (beta) gene]. Cells transfected with the TK gene alone transcribed very low levels of TK RNA. Cells cotransfected with plasmids bearing the sequences that encode the alpha-gene product infected cell protein 0 or 4 (ICP0 or ICP4) and the TK gene faithfully transcribed high levels of TK RNA. The plasmid containing the sequences encoding ICP0 was a more potent transactivator than the plasmid containing the sequences for ICP4.
使用HeLa细胞瞬时表达检测系统来确定从单纯疱疹病毒（HSV）中分离出的立即早期（α）基因是否能够转录激活1型（HSV-1）胸苷激酶（TK）基因[一种早期（β）基因]。单独转染TK基因的细胞转录出的TK RNA水平非常低。与携带编码α基因产物感染细胞蛋白0或4（ICP0或ICP4）序列的质粒和TK基因共转染的细胞忠实地转录出高水平的TK RNA。含有编码ICP0序列的质粒比含有ICP4序列的质粒是更有效的反式激活因子。
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