Smiley J R, Swan H, Pater M M, Pater A, Halpern M E
J Virol. 1983 Aug;47(2):301-10. doi: 10.1128/JVI.47.2.301-310.1983.
We examined the ability of deleted versions of the herpes simplex virus type 1 thymidine kinase gene, present in biochemically transformed mouse cells, to be induced in trans to a higher level of expression by superinfecting herpes simplex virus immediate early gene products. The results demonstrate that sequences mapping between -200 and -80 and between -70 and -12 are required for induction. As these regions are largely coincident with the previously identified thymidine kinase gene promoter, the results suggest that herpes simplex virus immediate early gene products or their metabolic product activate thymidine kinase expression by acting at the promoter region to increase the rate of transcription.
我们检测了存在于经生化转化的小鼠细胞中的1型单纯疱疹病毒胸苷激酶基因缺失版本，在被单纯疱疹病毒立即早期基因产物超感染时，被反式诱导至更高表达水平的能力。结果表明，诱导需要定位在-200至-80以及-70至-12之间的序列。由于这些区域与先前鉴定的胸苷激酶基因启动子基本重合，结果提示单纯疱疹病毒立即早期基因产物或其代谢产物通过作用于启动子区域来增加转录速率，从而激活胸苷激酶的表达。
