Starčević Kristina, Maurić Maja, Galan Asier, Gudan Kurilj Andrea, Mašek Tomislav
Faculty of Veterinary Medicine, Department of Animal Husbandry, University of Zagreb, Zagreb, Croatia.
Faculty of Veterinary Medicine, VetMedZg ERA Chair project, University of Zagreb, Zagreb, Croatia.
Andrologia. 2018 Oct;50(8):e13067. doi: 10.1111/and.13067. Epub 2018 Jun 19.
The aim of this study was to investigate the possible influence of different dietary n6/n3 ratios and DHA/EPA addition on the testis histology, antioxidative status and lipogenesis of streptozotocin-treated rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (55 mg/kg). The rats were divided into five groups: CON (n6/n3 ratio, 7), CON-DM1 (STZ; n6/n3 ratio, 7), N3-DM1 (STZ; n6/n3 ratio, 1), N6-DM1 (STZ; n6/n3 ratio, 60) and DHA-DM1 (STZ; n6/n3 ratio, 1; added DHA/EPA). Antioxidative status was improved in the DHA-DM1 group. Seminiferous tubule diameter, testicular pathohistological scoring and total lipid content were improved in the N6-DM1 group compared to the other streptozotocin-treated groups. Streptozotocin treatment induced changes in testis fatty acid profile depending on n6/n3 ratio. The fatty acid profile of N6-DM1 group was characterised by similar or increased values for n6 fatty acids compared to the CON group, while the DHA-DM1 group had the lowest content of n6 fatty acids. The content of n3 fatty acids was increased in the N3-DM1 and DHA-DM1 groups. These results suggest that a n6/n3 ratio could significantly influence testicular antioxidative status, histology and lipogenesis and that these effects vary depending on the supplemented fatty acid.
本研究的目的是调查不同饮食n6/n3比例以及添加DHA/EPA对链脲佐菌素处理大鼠的睾丸组织学、抗氧化状态和脂肪生成的可能影响。通过单次腹腔注射链脲佐菌素（55 mg/kg）诱导糖尿病。将大鼠分为五组：CON（n6/n3比例为7）、CON-DM1（链脲佐菌素；n6/n3比例为7）、N3-DM1（链脲佐菌素；n6/n3比例为1）、N6-DM1（链脲佐菌素；n6/n3比例为60）和DHA-DM1（链脲佐菌素；n6/n3比例为1；添加DHA/EPA）。DHA-DM1组的抗氧化状态得到改善。与其他链脲佐菌素处理组相比，N6-DM1组的生精小管直径、睾丸病理组织学评分和总脂质含量有所改善。链脲佐菌素处理根据n6/n3比例诱导睾丸脂肪酸谱发生变化。与CON组相比，N6-DM1组的n6脂肪酸值相似或增加，而DHA-DM1组的n6脂肪酸含量最低。N3-DM1组和DHA-DM1组的n3脂肪酸含量增加。这些结果表明，n6/n3比例可显著影响睾丸的抗氧化状态、组织学和脂肪生成，且这些影响因补充的脂肪酸不同而有所差异。
