Felber B K, Paskalis H, Kleinman-Ewing C, Wong-Staal F, Pavlakis G N
Science. 1985 Aug 16;229(4714):675-9. doi: 10.1126/science.2992082.
Expression of the pX protein of human T-cell leukemia virus type I (HTLV-I) in animal cells demonstrates that this protein is a specific transcriptional activator of the long terminal repeats (LTR) of HTLV-I. Several other promoters are not affected by pX. No lymphocyte-specific factors are required for this activation. pX can be detected in the nucleus of transfected monkey kidney cells (line CV1) by indirect immunofluorescence. These results indicate that the pX protein is essential for the replication cycle of the virus and that it may be directly involved in the immortalization of human lymphocytes by HTLV-I.
I型人类T细胞白血病病毒（HTLV-I）的pX蛋白在动物细胞中的表达表明，该蛋白是HTLV-I长末端重复序列（LTR）的特异性转录激活因子。其他几个启动子不受pX影响。这种激活不需要淋巴细胞特异性因子。通过间接免疫荧光法可在转染的猴肾细胞（CV1系）细胞核中检测到pX。这些结果表明，pX蛋白对病毒的复制周期至关重要，并且它可能直接参与HTLV-I对人类淋巴细胞的永生化过程。
