Matsunami N, Siomi H, Hatanaka M, Yaoita Y, Honjo T
Nucleic Acids Res. 1986 Jun 25;14(12):4779-86. doi: 10.1093/nar/14.12.4779.
The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their transcription activities in cell-free extracts of four cell lines; HeLa, CESS (Epstein-Barr virus-transformed human B cell line), MT-1 (HTLV-I-infected human T cell line without viral protein synthesis), and MT-2 (HTLV-I-infected human T cell line producing viral proteins). LTR was preferentially transcribed in the extracts of MT-2 although the other three genes were transcribed with relatively constant efficiencies in different extracts. The results agree well with the previous in vivo studies on the promoter activity of HTLV-I LTR. Mixing of HeLa and MT-2 extracts revealed the presence of a LTR-specific stimulating activity in MT-2 extracts.
对腺病毒2型主要晚期基因、小鼠β-珠蛋白基因、小鼠免疫球蛋白VH基因的启动子以及人I型嗜T淋巴细胞逆转录病毒的长末端重复序列（LTR），在四种细胞系的无细胞提取物中检测了它们的转录活性；这四种细胞系分别是：HeLa细胞系、CESS细胞系（爱泼斯坦-巴尔病毒转化的人B细胞系）、MT-1细胞系（感染了HTLV-I的人T细胞系，无病毒蛋白合成）和MT-2细胞系（感染了HTLV-I的人T细胞系，产生病毒蛋白）。尽管其他三个基因在不同提取物中以相对恒定的效率进行转录，但LTR在MT-2提取物中被优先转录。这些结果与之前关于HTLV-I LTR启动子活性的体内研究结果非常吻合。HeLa提取物与MT-2提取物混合后，发现在MT-2提取物中存在一种LTR特异性刺激活性。
