Sicard B, Lagarde M
Thromb Haemost. 1985 Apr 22;53(2):264-7.
Primary cultures of endothelial cells from human umbilical veins were grown until confluency. Then, dihomogammalinolenic acid (DHLA or 20:3n-6) and eicosapentaenoic acid (EPA or 20:5n-3), precursors of monoenoic and trienoic prostanoids, respectively, as well as 5,8,11-eicosatrienoic acid (20:3n-9), and isomer of DHLA, were incorporated into endothelial lipids. DHLA-rich endothelial cells had a decreased capacity of prostacyclin production. By contrast EPA- or 20:3n-9-rich endothelial cells were comparable to controls in this respect. DHLA and EPA were efficiently acylated into cell phospholipids and triglycerides at the opposite of 20:3n-9. It is suggested that both DHLA and EPA could alter the liberation of endogenous arachidonic acid for prostacyclin synthesis but this might be counterbalanced in EPA-rich endothelial cells by PGI3 production. We conclude that DHLA enrichment of endothelial cell lipids may impair the possible beneficial effect of the acid upon platelet functions whereas that of EPA would not be modified.
人脐静脉内皮细胞的原代培养物生长至汇合状态。然后，将分别作为单烯类和三烯类前列腺素前体的二高γ-亚麻酸（DHLA或20:3n-6）和二十碳五烯酸（EPA或20:5n-3），以及5,8,11-二十碳三烯酸（20:3n-9，DHLA的异构体）掺入内皮细胞脂质中。富含DHLA的内皮细胞产生前列环素的能力降低。相比之下，富含EPA或20:3n-9的内皮细胞在这方面与对照相当。与20:3n-9相反，DHLA和EPA有效地酰化进入细胞磷脂和甘油三酯。提示DHLA和EPA均可改变内源性花生四烯酸用于前列环素合成的释放，但在富含EPA的内皮细胞中，这可能被前列环素I3（PGI3）的产生所抵消。我们得出结论，内皮细胞脂质中富含DHLA可能损害该酸对血小板功能的潜在有益作用，而富含EPA则不会改变。
