Liu Yan-Yan, Chen Xing-Ru, Gao Ling-Fei, Chen Mo, Cui Wen-Qiang, Ding Wen-Ya, Chen Xue-Ying, God'spower Bello-Onaghise, Li Yan-Hua
College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China.
Front Pharmacol. 2018 Jun 5;9:570. doi: 10.3389/fphar.2018.00570. eCollection 2018.
Lindl. () has been used in herbal medicines for treating bacterial diseases. It is also thought to inhibit () biofilm formation. However, due to the inherent nature of the complexity in its chemical properties, it is difficult to understand the possible bioactive ingredients of The spectrum-effect relationships method was applied to screen the main active ingredients in obtained from Heilongjiang Province based on gray relational analysis. The results revealed that Sub-MICs obtained from 10 batches of could inhibit biofilm formation by . Gray relational analysis revealed variations in the contents of 15 main peaks and rutin was discovered to be the main active ingredient. Then, the function of rutin was further verified by inhibiting biofilm formation using crystal violet staining. Computational studies revealed that rutin may target the chloramphenicol acetyltransferase protein in the biofilm formation of In conclusion, this study revealed that the spectrum-effect relationships and computational studies are useful tools to associate the active ingredient with the potential anti-biofilm effects of . Here, our findings would provide foundation for the further understanding of the mechanism of intervention in biofilm formation.
林德尔（Lindl.）已被用于治疗细菌性疾病的草药中。它也被认为可以抑制（）生物膜的形成。然而，由于其化学性质复杂的固有特性，很难了解（）可能的生物活性成分。基于灰色关联分析，采用谱效关系法筛选了从黑龙江省获得的（）中的主要活性成分。结果表明，从10批次的（）中获得的亚抑菌浓度可以抑制（）生物膜的形成。灰色关联分析揭示了15个主峰含量的变化，发现芦丁是主要活性成分。然后，通过结晶紫染色抑制（）生物膜形成进一步验证了芦丁的功能。计算研究表明，芦丁可能靶向（）生物膜形成中的氯霉素乙酰转移酶蛋白。总之，本研究表明谱效关系和计算研究是将活性成分与（）潜在的抗生物膜作用联系起来的有用工具。在此，我们的研究结果将为进一步了解（）干预生物膜形成的机制提供基础。
