Chen Xing-Ru, Liu Yan-Yan, Zhou Yong-Hui, Xing Xiao-Xu, Qu Qian-Wei, Chen Xue-Ying, Ding Wen-Ya, Cheng Guang-Long, Wei Ai-Juan, Feng Xi-Wen, God'spower Bello-Onaghise, Eliphaz Nsabimana, Li Yan-Hua
College of Veterinary Medicine, Northeast Agricultural University 600 Changjiang Road, Xiangfang Harbin Heilongjiang 150030 P. R. China
Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development Harbin China.
RSC Adv. 2019 Nov 5;9(62):36088-36096. doi: 10.1039/c9ra06224f. eCollection 2019 Nov 4.
Lindl. () is a medicinal plant with effective broad-spectrum antibacterial activity, which can also inhibit biofilm formation. The processing of herbal medicine can purify medicinal materials, provide acceptable taste, reduce toxicity, enhance efficacy, influence performance and facilitate preparation. Thus, the aim of this study was to enhance the biofilm inhibition activity of toward () using the best processing method. The content of rutin and flavonoids and the ability to inhibit the biofilm formation by were examined using four processing methods. One of the best methods, the process of stir-frying with vinegar, was optimized based on the best rutin content by response surface methodology. The histidine content and gene expression of biofilm , resulting from stir-frying with vinegar, were evaluated and were found to be significantly decreased and down-regulated, respectively. The results show that stir-fried with vinegar can be used to effectively treat diseases resulting from infection. This is because it significantly inhibited biofilm formation by interfering with the biosynthesis of histidine; thus, its mechanism of action is decreasing histidine synthesis.
林德尔氏（植物名，此处原文未完整给出具体植物学名）是一种具有有效广谱抗菌活性的药用植物，它还能抑制生物膜形成。中药炮制可以提纯药材、提供可接受的口感、降低毒性、增强药效、影响性能并便于制剂。因此，本研究的目的是使用最佳炮制方法提高林德尔氏（植物名）对（此处原文未完整给出具体对象）的生物膜抑制活性。采用四种炮制方法检测了芦丁和黄酮类化合物的含量以及林德尔氏（植物名）抑制生物膜形成的能力。基于最佳芦丁含量，通过响应面法对最佳方法之一——醋炒林德尔氏（植物名）的工艺进行了优化。对醋炒林德尔氏（植物名）导致的生物膜中组氨酸含量和（此处原文未完整给出具体基因名）基因表达进行了评估，发现分别显著降低和下调。结果表明，醋炒林德尔氏（植物名）可用于有效治疗由（此处原文未完整给出具体对象）感染引起的疾病。这是因为它通过干扰组氨酸的生物合成显著抑制了（此处原文未完整给出具体对象）生物膜的形成；因此，其作用机制是减少组氨酸的合成。
