School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University , Shenyang , China.
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Wuya College of Innovation, Shenyang Pharmaceutical University , Shenyang , China.
Nat Prod Res. 2019 Dec;33(24):3527-3532. doi: 10.1080/14786419.2018.1487967. Epub 2018 Jun 20.
The fruit of , a kind of mainly medicinal plant, possesses good antitumor properties. In order to explore the bioactive compounds for the treatment of hepatocellular carcinoma, the study focused on the isolation of cytotoxic compounds from the fruit of , which led to the discovery of fourteen compounds, including one new triterpene, 3,20-dihydroxy-30-methyl,17(29)--epoxy-28-norlupane (), together with thirteen known compounds (-). The structures of isolated compounds were demonstrated by spectroscopic methods including 1D and 2D NMR spectroscopy. Moreover, all triterpenes were evaluated for antiproliferative activities against two human hepatocellular carcinoma cell lines, HepG2 and Hep3B. Compound showed the strongest cytotoxic activity against the HepG2 with IC value at 29.6 μM. Further study demonstrated that compound exhibited cytotoxic activity through the induction of apoptosis.
，一种主要的药用植物，其果实具有良好的抗肿瘤特性。为了探索治疗肝癌的生物活性化合物，本研究专注于从果实中分离细胞毒性化合物，从而发现了 14 种化合物，包括一种新的三萜，3,20-二羟基-30-甲基，17(29)--环氧-28-降齐墩烷（），以及 13 种已知化合物（-）。通过包括 1D 和 2D NMR 光谱在内的光谱方法证明了分离化合物的结构。此外，所有三萜类化合物均针对两种人肝癌细胞系 HepG2 和 Hep3B 的增殖活性进行了评估。化合物对 HepG2 的细胞毒性活性最强，IC 值为 29.6 μM。进一步的研究表明，化合物通过诱导细胞凋亡发挥细胞毒性活性。
