First, third, eighth, ninth, tenth, thirteenth, and eighteenth authors: Department of Plant Pathology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611; first, second, third, fourth, fifth, sixth, seventh, and eighteenth authors: North Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Quincy 32351; seventh, twelfth, and seventeenth authors: Horticultural Sciences Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville; eleventh author: Institute of Food and Agricultural Sciences, Statistics Division, University of Florida, Gainesville; twelfth, thirteenth, and seventeenth authors: Gulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma 33598; fourteenth author: The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK; and fifteenth and sixteenth authors: Two Blades Foundation, Evanston, IL, 60201.
Phytopathology. 2018 Dec;108(12):1402-1411. doi: 10.1094/PHYTO-12-17-0424-R. Epub 2018 Oct 24.
Field trials were conducted at two locations in Florida to evaluate transgenic tomato expressing the ELONGATION FACTOR TU RECEPTOR (EFR) gene from Arabidopsis thaliana, the Bs2 gene from pepper, or both Bs2 and EFR (Bs2/EFR) for managing bacterial wilt caused by Ralstonia solanacearum and bacterial spot caused by Xanthomonas perforans. Expression of EFR or Bs2/EFR in the susceptible genotype Fla. 8000 significantly reduced bacterial wilt incidence (50 to 100%) and increased total yield (57 to 114%) relative to lines expressing only Bs2 or the nontransformed Fla. 8000 control, although the marketable yield was not significantly affected. Following harvest, surviving symptomatic and nonsymptomatic plants were assessed for colonization by R. solanacearum. There were no significant differences in the population at the lower stem. Interestingly, in the middle stem, no bacteria could be recovered from EFR or Bs2/EFR lines but viable bacterial populations were recovered from Bs2 and nontransformed control lines at 10 to 10 CFU/g of stem tissue. In growth-chamber experiments, the EFR transgenic tomato lines were found to be effective against seven different R. solanacearum strains isolated from the southeastern United States, indicating utility across the southeastern United States. In all of the bacterial spot trials, EFR and Bs2/EFR lines had significantly reduced disease severity (22 to 98%) compared with the Fla. 8000 control. The marketable and total yield of Bs2/EFR were significantly higher (43 to 170%) than Fla. 8000 control in three of four field trials. These results demonstrate for the first time the potential of using the EFR gene for field management of bacterial wilt and bacterial spot diseases of tomato.
在佛罗里达州的两个地点进行了田间试验，以评估表达来自拟南芥的伸长因子 TU 受体（EFR）基因、来自辣椒的 Bs2 基因或同时表达 Bs2 和 EFR（Bs2/EFR）的转基因番茄，用于防治由罗尔斯顿氏菌引起的细菌性萎蔫病和由黄单胞菌穿孔引起的细菌性斑点病。在易感基因型 Fla. 8000 中表达 EFR 或 Bs2/EFR 与仅表达 Bs2 或未转化的 Fla. 8000 对照相比，显著降低了细菌性萎蔫病的发病率（50%至 100%）并增加了总产量（57%至 114%），尽管商品产量没有受到显著影响。收获后，对存活的有症状和无症状植物进行了罗尔斯顿氏菌的定殖评估。在较低的茎部，种群没有显著差异。有趣的是，在中间茎部，无法从 EFR 或 Bs2/EFR 系中回收细菌，但可以从 Bs2 和未转化的对照系中回收存活的细菌种群，其在茎组织中的含量为 10 至 10 CFU/g。在生长室实验中，发现 EFR 转基因番茄系对从美国东南部分离的七种不同的罗尔斯顿氏菌菌株有效，表明在美国东南部具有广泛的应用潜力。在所有的细菌性斑点试验中，与 Fla. 8000 对照相比，EFR 和 Bs2/EFR 系的病情严重程度显著降低（22%至 98%）。在四个田间试验中的三个试验中，Bs2/EFR 的商品产量和总产量（43%至 170%）均显著高于 Fla. 8000 对照。这些结果首次证明了使用 EFR 基因对番茄细菌性萎蔫病和细菌性斑点病进行田间管理的潜力。
