Two Blades Foundation, Evanston, Illinois, United States of America.
PLoS One. 2012;7(8):e42036. doi: 10.1371/journal.pone.0042036. Epub 2012 Aug 1.
We investigated whether lines of transgenic tomato (Solanum lycopersicum) expressing the Bs2 resistance gene from pepper, a close relative of tomato, demonstrate improved resistance to bacterial spot disease caused by Xanthomonas species in replicated multi-year field trials under commercial type growing conditions. We report that the presence of the Bs2 gene in the highly susceptible VF 36 background reduced disease to extremely low levels, and VF 36-Bs2 plants displayed the lowest disease severity amongst all tomato varieties tested, including commercial and breeding lines with host resistance. Yields of marketable fruit from transgenic lines were typically 2.5 times that of the non-transformed parent line, but varied between 1.5 and 11.5 fold depending on weather conditions and disease pressure. Trials were conducted without application of any copper-based bactericides, presently in wide use despite negative impacts on the environment. This is the first demonstration of effective field resistance in a transgenic genotype based on a plant R gene and provides an opportunity for control of a devastating pathogen while eliminating ineffective copper pesticides.
我们研究了表达来自胡椒（番茄的近亲）的 Bs2 抗性基因的转基因番茄品系在商业种植条件下的复发性多年田间试验中对由黄单胞菌属引起的细菌性斑点病是否具有更好的抗性。我们报告说，在高度易感的 VF 36 背景下存在 Bs2 基因将疾病降低到极低水平，并且 VF 36-Bs2 植物在所有测试的番茄品种中表现出最低的疾病严重程度，包括具有宿主抗性的商业和育种品系。转基因品系的可销售水果产量通常是非转化亲本系的 2.5 倍，但根据天气条件和疾病压力的不同，产量在 1.5 到 11.5 倍之间变化。试验是在不使用任何铜基杀真菌剂的情况下进行的，尽管这些杀菌剂对环境有负面影响，但目前仍在广泛使用。这是首次基于植物 R 基因在转基因基因型中有效田间抗性的证明，为控制毁灭性病原体提供了机会，同时消除了无效的铜农药。
