Czerkawski J W, Breckenridge G
Arch Tierernahr. 1985 Apr;35(4):261-77. doi: 10.1080/17450398509424712.
Long-term experiments were made with the Rumen Simulation Technique (Rusitec) with hay diet and with three different protein supplements (casein, fish meal and soyabean meal). Soyabean meal made the greatest contribution to the output of end-products of fermentation and casein made the smallest contribution. The supplements did not have any marked effect on the metabolism of the basal diet of hay. The experiments showed that rumen microorganisms destroy a large proportion of protein (measured as total alpha-NH2 groups) and that this loss of protein was increased by supplementation with high protein compounds. The degradation of protein in soyabean meal and fish meal appeared to be considerably greater in the model system than the reported results of measurement in vivo and in situ.
采用瘤胃模拟技术（Rusitec）对干草日粮以及三种不同蛋白质补充剂（酪蛋白、鱼粉和豆粕）进行了长期试验。豆粕对发酵终产物的产量贡献最大，酪蛋白的贡献最小。这些补充剂对干草基础日粮的代谢没有显著影响。试验表明，瘤胃微生物会破坏很大一部分蛋白质（以总α-NH2基团衡量），并且补充高蛋白化合物会增加这种蛋白质损失。在模型系统中，豆粕和鱼粉中蛋白质的降解似乎比体内和原位测量的报道结果要大得多。
