Coelho Polyana Galvão Bernardes, Souza Maria Verônica de, Conceição Lissandro Gonçalves, Viloria Marlene Isabel Vargas, Bedoya Sirley Adriana Ortiz
Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
An Bras Dermatol. 2018 Jun;93(3):415-418. doi: 10.1590/abd1806-4841.20187544.
The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture images of histological sections stained with picrosirius red and evaluated under polarized light microscopy, as well as possible artefacts that may occur.
特殊的天狼星红苦味酸染色突出了胶原纤维在偏振光下的天然双折射现象。双折射结果有助于评估组织中胶原纤维的排列情况。作者旨在阐明获取和拍摄用天狼星红苦味酸染色并在偏振光显微镜下评估的组织学切片图像的所有步骤，以及可能出现的伪像。
