Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), Bertrand Russel Av., Carl Von Linnaeus Street., Campinas, São Paulo, Brazil.
Laboratory of Microscopy and Microanalysis, Department of Biology, São Paulo State University (UNESP), Cristóvão Colombo St., São José Do Rio Preto, São Paulo, 2265, Brazil.
Histochem Cell Biol. 2023 Nov;160(5):419-433. doi: 10.1007/s00418-023-02223-3. Epub 2023 Jul 20.
Telocytes are interstitial cells that are present in various tissues, have long cytoplasmic projections known as telopodes, and are classified as CD34 cells. Telopodes form extensive networks that permeate the stroma, and there is evidence that these networks connect several stromal cell types, giving them an important role in intercellular communication and the maintenance of tissue organisation. Data have also shown that these networks can be impaired and the number of telocytes reduced in association with many pathological conditions such as cancer and fibrosis. Thus, techniques that promote telocyte proliferation have become an important therapeutic target. In this study, ex vivo and in vitro assays were conducted to evaluate the impact on prostatic telocytes of SDF-1, a factor involved in the proliferation and migration of CD34 cells. SDF-1 caused an increase in the number of telocytes in explants, as well as morphological changes that were possibly related to the proliferation of these cells. These changes involved the fusion of telopode segments, linked to an increase in cell body volume. In vitro assays also showed that SDF-1 enriched prostate stromal cells with telocytes. Altogether, the data indicate that SDF-1 may offer promising uses in therapies that aim to increase the number of telocytes. However, further studies are needed to confirm the efficiency of this factor in different tissues/pathological conditions.
间质细胞是存在于各种组织中的细胞，具有称为 telopodes 的长细胞质突起，被归类为 CD34 细胞。Telopodes 形成广泛的网络，渗透到基质中，有证据表明这些网络连接几种基质细胞类型，使它们在细胞间通讯和组织组织维持中发挥重要作用。数据还表明，这些网络可能会受损，并且与许多病理状况（如癌症和纤维化）相关的 telocyte 数量会减少。因此，促进 telocyte 增殖的技术已成为重要的治疗目标。在这项研究中，进行了离体和体外测定，以评估 SDF-1（一种参与 CD34 细胞增殖和迁移的因子）对前列腺 telocyte 的影响。SDF-1 导致外植体中 telocyte 的数量增加，以及可能与这些细胞增殖有关的形态变化。这些变化涉及 telopode 段的融合，与细胞体体积的增加有关。体外测定还表明，SDF-1 使富含 telocyte 的前列腺基质细胞增多。总的来说，这些数据表明 SDF-1 可能在旨在增加 telocyte 数量的治疗中具有广阔的应用前景。然而，需要进一步的研究来确认该因子在不同组织/病理条件下的效率。
