García-Alvarado Francisco Javier, Neri-Meléndez Honorio, Pérez Armendáriz Luz, Rivera Guillen Mario
Centro de Investigación Biomédica, Universidad Autónoma de Coahuila. Coahuila, México.
Facultad de Medicina Torreón, Universidad Autónoma de Coahuila. Coahuila, México.
Rev Peru Med Exp Salud Publica. 2018 Jan-Mar;35(1):72-76. doi: 10.17843/rpmesp.2018.351.3565..
The aim of this study was to assess the relationship of the rs11191439 and rs3740393 polymorphisms of the Arsenite Methyltransferase (As3MT) gene with the arsenic urinary excretion profiles in a population in northern Mexico. A crosssectional study was conducted with 102 participants between the ages of 14 and 75 in Comarca Lagunera, Mexico. Speciation of the arsenic urinary metabolites was determined, and DNA was extracted; analysis and amplification of the polymorphisms was made by PCR in real time. A descriptive analysis was carried out and the differences in the urinary arsenic concentrations were assessed according to sex and genotype. The median concentration of arsenic in water was 82 μg/L; the levels of urinary excretion of dimethylarsinic acid (DMA) were higher in women than in the men. The carriers of the CC genetic variant of the As3MT (rs3740393) gene showed higher urinary concentrations of methylarsinic acid (p=0.01) and DMA (p=0.05).
本研究的目的是评估墨西哥北部人群中亚砷酸盐甲基转移酶（As3MT）基因的rs11191439和rs3740393多态性与尿砷排泄谱之间的关系。在墨西哥拉古内拉地区对102名年龄在14至75岁之间的参与者进行了一项横断面研究。测定了尿砷代谢产物的形态，并提取了DNA；通过实时PCR对多态性进行分析和扩增。进行了描述性分析，并根据性别和基因型评估了尿砷浓度的差异。水中砷的中位浓度为82μg/L；女性二甲基砷酸（DMA）的尿排泄水平高于男性。As3MT（rs3740393）基因CC基因变体的携带者尿中甲基砷酸（p = 0.01）和DMA（p = 0.05）的浓度较高。
