Yason C V, Schat K A
Avian Dis. 1985 Apr-Jun;29(2):499-508.
Rotaviruses were isolated from intestinal contents obtained from flocks of turkey poults and pheasant poults with diarrhea and from different age groups of chickens showing various signs of intestinal disorders. The incorporation of 5 micrograms trypsin/ml in the inoculum and medium was essential for virus isolation in chicken kidney cells. All isolates were identified as rotaviruses by fluorescent-antibody technique using a National Institutes of Health reference rotavirus antiserum against human rotavirus strain "D," Type 2. Negative-staining and transmission electron microscopy showed the presence of rotavirus particles. Furthermore, polyacrylamide gel electrophoresis pattern of viral RNA segments of our isolates confirmed that they are rotaviruses. Seven-day-old turkey poults could be infected with turkey and chicken rotavirus isolates; in contrast, chicks of the same age were refractory.
从患有腹泻的火鸡雏鸡和雉鸡雏鸡群的肠道内容物以及表现出各种肠道疾病症状的不同年龄组鸡中分离出轮状病毒。接种物和培养基中每毫升加入5微克胰蛋白酶对于在鸡肾细胞中分离病毒至关重要。使用美国国立卫生研究院针对人轮状病毒2型“D”株的参考轮状病毒抗血清，通过荧光抗体技术将所有分离株鉴定为轮状病毒。负染色和透射电子显微镜显示存在轮状病毒颗粒。此外，我们分离株的病毒RNA片段的聚丙烯酰胺凝胶电泳图谱证实它们是轮状病毒。7日龄的火鸡雏鸡可被火鸡和鸡轮状病毒分离株感染；相比之下，相同年龄的雏鸡则具有抗性。
