钒酰对大鼠肾上腺膜中腺苷酸环化酶激活反应的抑制作用。
Inhibition by vanadyl of adenylate cyclase activation reactions in rat adrenal membranes.
作者信息
Hayashi Y, Kimura T
出版信息
Biochem Biophys Res Commun. 1985 Aug 15;130(3):945-51. doi: 10.1016/0006-291x(85)91706-1.
In rat adrenal dispersed cells, both vanadyl and vanadate inhibited ACTH-stimulated steroidogenesis and the formation of cAMP, whereas these compounds did not inhibit the cAMP-dependent steroidogenesis. Then, the membrane fraction was prepared and activated by various secretagogues including ACTH, Gpp(NH)p, GTP gamma S, and forskolin. The cyclase activity was inhibited by vanadyl but not by vanadate in the presence of these stimulators. Based on these results, we conclude that cationic vanadyl acts against a metal-requiring step in the adenylate cyclase system containing G-protein and the catalytic subunit. In addition, we believe that vanadyl is a useful tool to investigate adenylate cyclase systems.
在大鼠肾上腺分散细胞中，氧钒根离子和钒酸盐均抑制促肾上腺皮质激素（ACTH）刺激的类固醇生成及环磷酸腺苷（cAMP）的形成，而这些化合物并不抑制cAMP依赖性类固醇生成。随后，制备了膜组分并用包括ACTH、鸟苷-5'-O-(3-硫代三磷酸)（Gpp(NH)p）、鸟苷-5'-O-(γ-硫代)三磷酸（GTPγS）和福斯可林在内的各种促分泌素进行激活。在这些刺激剂存在的情况下，氧钒根离子抑制环化酶活性，而钒酸盐则无此作用。基于这些结果，我们得出结论，阳离子氧钒根离子作用于含G蛋白和催化亚基的腺苷酸环化酶系统中一个需要金属的步骤。此外，我们认为氧钒根离子是研究腺苷酸环化酶系统的有用工具。
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