College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Bio-Pharmaceutical College, Beijing City University, Beijing 100094, China.
Molecules. 2018 Jun 20;23(6):1495. doi: 10.3390/molecules23061495.
In this experiment, corn stover was treated with optimal combined pretreatment conditions: 2% NaOH at 80 °C treated 2 h combined with initial pH 9 at the ozone concentration of 78 mg/mL treated 25 min. The effect of lignin removal rate on the enzymatic hydrolysis degree of cellulose during the treatment process was studied. At the same time, the lignin in the optimal pretreated corn stover was separated and extracted by enzymatic acidolysis, and its structure and connection were characterized. The results showed that the alkali combined with ozone pretreatment improved the enzymatic hydrolysis degree of the cellulose while exfoliating and degrading the macromolecular lignin into small molecules. The stable crosslink structure of the lignin-cellulose-hemicellulose was destroyed, and the lignocellulosic structure changed in favor of the enzymatic hydrolysis of the cellulose.
在本实验中，采用最佳组合预处理条件对玉米秸秆进行处理：2%的 NaOH 在 80°C 下处理 2 小时，与臭氧浓度为 78mg/mL 时初始 pH9 的条件相结合处理 25 分钟。研究了木质素去除率对处理过程中纤维素酶解程度的影响。同时，通过酶酸解将最佳预处理后的玉米秸秆中的木质素分离和提取，并对其结构和连接进行了表征。结果表明，碱结合臭氧预处理提高了纤维素的酶解程度，同时将大分子木质素解聚成小分子。木质素-纤维素-半纤维素的稳定交联结构被破坏，木质纤维素结构发生变化，有利于纤维素的酶解。
