Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, China (mainland).
Clinical Laboratory, First Affiliated Hospital of Guangdong Pharmaceutical University, Guongzhou, Guangdong, China (mainland).
Med Sci Monit. 2018 Jun 21;24:4254-4261. doi: 10.12659/MSM.908647.
BACKGROUND Cytokeratin 19 (CK19) is a typical epithelial marker. In this study, we determined whether epidermal growth factor (EGF) or basic fibroblast growth factor (bFGF) could enhance CK19 expression in adipose-derived stem cells (ADSCs), thereby inducing the differentiation of ADSCs into epithelial-like cells. MATERIAL AND METHODS ADSCs were isolated from perinephric fat, and the expression of CD29, CD90, and CD105 was confirmed. Following isolation, ADSCs were cultured in static medium or medium containing EGF or bFGF. RESULTS Flow cytometry revealed that EGF and bFGF could alter mesenchymal stem cell markers as well as the cell cycle of ADSCs. Western blotting and immunofluorescence revealed that after 14 days, EGF treatment enhanced the expression of CK19 in ADSCs. CONCLUSIONS Our findings offer important insight for the clinical use of ADSCs in the generation of epithelial-like cells in the future.
细胞角蛋白 19（CK19）是一种典型的上皮细胞标志物。本研究旨在确定表皮生长因子（EGF）或碱性成纤维细胞生长因子（bFGF）是否能增强脂肪来源干细胞（ADSCs）中 CK19 的表达，从而诱导 ADSCs 向上皮样细胞分化。
从肾周脂肪中分离 ADSC，并确认 CD29、CD90 和 CD105 的表达。分离后，将 ADSC 培养在静态培养基或含 EGF 或 bFGF 的培养基中。
流式细胞术显示，EGF 和 bFGF 可以改变间充质干细胞标志物和 ADSC 的细胞周期。Western blot 和免疫荧光显示，EGF 处理 14 天后增强了 CK19 在 ADSC 中的表达。
我们的研究结果为未来 ADSC 在生成上皮样细胞中的临床应用提供了重要的见解。
