Mitchell Cancer Institute, University of South Alabama, 1660 Springhill Ave., Mobile, AL, 36604, USA.
Eastern Virginia Medical School, Leroy T. Canoles Jr. Cancer Research Center, Lester Hall 651 Colley Ave. Rm. 429, Norfolk, VA, 23501, USA.
Glycoconj J. 2018 Jun;35(3):333-342. doi: 10.1007/s10719-018-9828-5. Epub 2018 Jun 9.
A promising approach capitalizing on the specific and highly sensitive characteristics of the body's own immune system is demonstrated in the context of revealing pancreatic ductal adenocarcinoma cancer (PDAC). IgA from a local biofluid called gastrointestinal lavage fluid (GLF) is used to investigate glycan reactivity to show the potential of this approach. IgA antibody responses, just as with IgG, result in amplification of a small signal which aids in detecting changes from a healthy state. IgA from GLF was screened against glycan arrays containing 609 glycan structures to investigate differential binding patterns associated with the disease. Samples included PDAC (n = 14) and non-PDAC (n = 6). Non-PDAC conditions included samples from healthy patients and the potentially confounding conditions of colon cancer and its precancerous lesion, colon adenoma. Results demonstrated characteristic reactivity in the PDAC sample group to a glycan structure. Also, IgA non-reactive motifs arose showing remarkable consistency within and between sample groups. While sample sizes are too small to identify putative biomarkers, these data show the use of IgA from GLF to be a promising avenue of research for local disease biomarker discovery.
一种有前景的方法利用了人体自身免疫系统的特异性和高度敏感性特征，在揭示胰腺导管腺癌（PDAC）的过程中得到了证明。利用一种名为胃肠灌洗液（GLF）的局部生物流体中的 IgA 来研究聚糖反应性，以展示这种方法的潜力。与 IgG 一样，IgA 抗体反应导致小信号的放大，有助于检测健康状态的变化。GLF 的 IgA 与包含 609 种聚糖结构的聚糖阵列进行筛选，以研究与疾病相关的差异结合模式。样本包括 PDAC（n=14）和非 PDAC（n=6）。非 PDAC 条件包括来自健康患者的样本以及结肠癌及其癌前病变结肠腺瘤的潜在混杂条件。结果表明，PDAC 样本组对一种聚糖结构表现出特征性反应。此外，出现了 IgA 非反应性基序，在样本组内和组间表现出显著的一致性。虽然样本量太小，无法确定潜在的生物标志物，但这些数据表明，使用 GLF 中的 IgA 是发现局部疾病生物标志物的有前途的研究途径。
