Acquotti Domenico, Mauri Laura, Sonnino Sandro
Centro Interdipartimentale Misure, University of Parma, Parma, Italy.
Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy.
Methods Mol Biol. 2018;1804:241-284. doi: 10.1007/978-1-4939-8552-4_12.
Structure, conformation, and dynamics of sphingolipids can provide substantial help in better understanding sphingolipid-ligand interaction mechanisms. Both the oligosaccharide structure and the ceramide moiety of native glycosphingolipid can be established directly by NMR spectroscopic analysis without the necessity to resort to any other chemical or spectroscopic methods. NMR is a powerful technique to investigate interaction between small ligand, such as ganglioside, and membrane protein.
鞘脂的结构、构象和动力学有助于更好地理解鞘脂-配体相互作用机制。天然糖鞘脂的寡糖结构和神经酰胺部分都可以通过核磁共振光谱分析直接确定，无需借助任何其他化学或光谱方法。核磁共振是研究神经节苷脂等小分子配体与膜蛋白之间相互作用的有力技术。
