蒲公英多糖对溃疡性结肠炎大鼠IL-6/STAT3信号通路的影响

[Effects of Dandelion polysaccharide on IL-6/STAT3 signaling pathway in ulcerative colitis rats].

作者信息

Wang Qian, Bie Yu-Long, Wang Dou, Fan Wen-Tao

机构信息

Shanxi University of Chinese Medicine, Xianyang 712046, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 May 8;33(5):422-425. doi: 10.12047/j.cjap.5525.2017.102.

DOI:10.12047/j.cjap.5525.2017.102

PMID:29926586

Abstract

OBJECTIVE

To investigate the effects of dandelion polysaccharide on IL-6/STAT3 signaling pathway in rats with ulcerative colitis.

METHODS

Forty SD rats were randomly divided into four groups (=10):control group, model group, positive control group and dandelion polysaccharide group. The ulcerative colitis model was established by treated with 2,4,6-trinitrobenzene sulfonic acid (TNBS). The positive control group was treated with mesalazine 10 mg/kg·d and dandelion polysaccharide group was treated with dandelion polysaccharide 10 mg/kg·d. The levels of interleukin -6(IL-6), colonic myeloperoxidase (MPO) and interleukin-6 receptor (sIL-6Rα) were measured after 4 weeks of treatment. The pathological changes of colonic mucosa were observed in rats. The gene expressions of glycoprotein 130 (gp130), transcriptional activator3(STAT3) and IL-6 were detected.

RESULTS

Compared with the normal control group, the level of serum IL-6 in the model group was significantly higher than that in the control group (<0.01). Compared with the model group, the serum levels of IL-6 in the dandelion polysaccharide group and the methacetin group were significantly decreased (<0.01). Compared with the model group, the MPO positive density of the rats in the dandelion polysaccharide group and the methacetin group was significantly lower than that in the normal group (<0.01). Compared with the model group, the levels of sIL-6Rα and gp130 in the rats were significantly lower than those in the model group (<0.01). Compared with the model group, the expressions of STAT3 and IL-6 mRNA in the intestinal tissue of the rats in the dandelion polysaccharide group and the methacetin group were decreased significantly.

CONCLUSIONS

Dandelion polysaccharide can decrease the level of IL-6 in rats with ulcerative colitis, regulate the expression of sIL-6Rα and gp130 protein in IL-6/STAT3 pathway, and then down-regulate the expressions of STAT3 and IL-6 mRNA in intestinal tissue of rats, alleviate the colon inflammation state, protect and repair the mucosal tissue. Dandelion polysaccharide plays a role in the treatment of ulcerative colitis.

摘要

目的

探讨蒲公英多糖对溃疡性结肠炎大鼠白细胞介素-6（IL-6）/信号转导与转录激活因子3（STAT3）信号通路的影响。

方法

将40只SD大鼠随机分为4组（每组10只）：对照组、模型组、阳性对照组和蒲公英多糖组。采用2,4,6-三硝基苯磺酸（TNBS）制备溃疡性结肠炎模型。阳性对照组给予美沙拉嗪10 mg/kg·d，蒲公英多糖组给予蒲公英多糖10 mg/kg·d。治疗4周后检测白细胞介素-6（IL-6）、结肠髓过氧化物酶（MPO）及白细胞介素-6受体（sIL-6Rα）水平，观察大鼠结肠黏膜病理变化，检测糖蛋白130（gp130）、转录激活因子3（STAT3）及IL-6基因表达。

结果

与正常对照组比较，模型组血清IL-6水平显著升高（<0.01）。与模型组比较，蒲公英多糖组和美沙拉嗪组血清IL-6水平显著降低（<0.01）。与模型组比较，蒲公英多糖组和美沙拉嗪组大鼠MPO阳性密度显著低于模型组（<0.01）。与模型组比较，大鼠sIL-6Rα和gp130水平显著低于模型组（<0.01）。与模型组比较，蒲公英多糖组和美沙拉嗪组大鼠肠道组织中STAT3和IL-6 mRNA表达显著降低。