β-肾上腺素能受体拮抗剂SR59230A对大鼠胸主动脉张力及微小RNA表达的影响

[Effect of β-AR antagonist SR59230A on the tension and microRNA expression of rat thoracic aorta].

作者信息

Zhao Qian-Qian, Jing Jia-Ni, Li Hai-Qing, Liu Rong, Li Xiao-Peng, Cui Xiang-Li

机构信息

Department of Physiology, Cell Physiology Laboratory, Shanxi Medical University, Taiyuan 030001, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Jan 8;33(1):6-10. doi: 10.12047/j.cjap.5448.2017.002.

DOI:10.12047/j.cjap.5448.2017.002

PMID:29926599

Abstract

OBJECTIVE

To explore the effects of SR59230A on the tension and microRNA (miRNA) expression of rat thoracic aorta.

METHODS

Forty-four SD rats were used in the experiment. Twenty-four rats were used to observe the effect of SR on the tension of thoracic aortic rings. Another 20 rats were randomly divided into control (=10) and SR group(=10). Rats in SR group were injected SR intraperitoneally,and in control group were given 0.9% of saline. After 5 weeks, the blood pressure of all rats were measured. Then the tension to NA and the expression of miRNA of thoracic aorta rings were measured.

RESULTS

(1) The tension of thoracic aortic rings responding to 30 mmol/LKCl were increased by pretreatment of SR (<0.05); (2) After 5 weeks injection of SR, systolic pressure was increased (<0.05); (3) The tension in SR group was increased in presence of 1 mol/L and 10 mol/L of NA (<0.05,<0.01). (4) After 5 weeks of SR in vivo application,18 miRNA were down-regulated, 7 of them had statistical significance, they were rno-miR-143-3p, rno-miR-29b-3p, rno-miR-31a-5p, rno-let-7b-5p, rno-miR-214-3p, rno-miR-222-3p and rno-miR-352; 11 miRNA were up-regulated, 4 of them had statistical significance, they were rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p and rno-miR-499-5p respectively.

CONCLUSIONS

SR59230A increased the tension of rat thoracic aorta. In vivo administration of SR led to increase of systolic pressure of rat,down-regulation of rno-miR-143-3p、rno-miR-29b-3p、rno-miR-31a-5p、rno-let-7b-5p、rno-miR-214-3p、rno-miR-222-3p、rno-miR-352 and up-regulation of rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p and rno-miR-499-5p.

摘要

目的

探讨SR59230A对大鼠胸主动脉张力及微小RNA（miRNA）表达的影响。

方法

实验选用44只SD大鼠。24只大鼠用于观察SR对胸主动脉环张力的影响。另外20只大鼠随机分为对照组（n = 10）和SR组（n = 10）。SR组大鼠腹腔注射SR，对照组给予0.9%生理盐水。5周后，测量所有大鼠的血压。然后检测胸主动脉环对去甲肾上腺素（NA）的张力及miRNA的表达。

结果

（1）SR预处理可增加胸主动脉环对30 mmol/L氯化钾（KCl）的张力（P<0.05）；（2）注射SR 5周后，收缩压升高（P<0.05）；（3）SR组在1 μmol/L和10 μmol/L NA作用下张力增加（P<0.05，P<0.01）。（4）体内应用SR 5周后，18种miRNA表达下调，其中7种具有统计学意义，分别为rno-miR-143-3p、rno-miR-29b-3p、rno-miR-31a-5p、rno-let-7b-5p、rno-miR-214-3p、rno-miR-222-3p和rno-miR-352；11种miRNA表达上调，其中4种具有统计学意义，分别为rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p和rno-miR-499-5p。

结论

SR59230A可增加大鼠胸主动脉张力。体内给予SR导致大鼠收缩压升高，rno-miR-143-3p、rno-miR-29b-3p、rno-miR-31a-5p、rno-let-7b-5p、rno-miR-214-3p、rno-miR-222-3p、rno-miR-352表达下调，rno-miR-206-3p、rno-miR-223-3p、rno-miR-342-3p和rno-miR-499-5p表达上调。