Parker Mitchell I, Palladino Michael A
Monmouth University, West Long Branch, NJ, USA.
Drexel University College of Medicine, Philadelphia, PA, USA.
Am J Reprod Immunol. 2017 Jun;77(6). doi: 10.1111/aji.12673. Epub 2017 Mar 22.
Little is known about how infection and the response to inflammation affect the microRNA (miRNA) profile of the male reproductive tract. We hypothesized that expression of inflammatory-related miRNAs would be altered following immune activation of rat testis.
Testis total RNA was purified from Sprague-Dawley rats 3 or 6 hours after receiving a 5 mg/kg intraperitoneal injection of bacterial lipopolysaccharide (LPS) and examined by qPCR using an 84-panel miRNA array.
Five inflammatory-related miRNAs showed a greater than twofold downregulation (P<.05) in the 3-hour group (rno-let-7f-5p, rno-miR-200c-3p, rno-miR-23a-3p, rno-miR-23b-3p, rno-miR-98-5p) and five from the 6-hour group (rno-miR-17-5p, rno-miR-19a-3p, rno-miR-34a-5p, rno-miR-34c-5p, rno-miR-449a-5p).
Review of the literature has revealed that these miRNAs also play important roles in the maintenance of fertility, formation and elimination of cancer, and development of the male reproductive tract. Further study will lead to a greater understanding of male reproductive immunology and related health issues.
关于感染及炎症反应如何影响雄性生殖道的微小RNA（miRNA）谱，我们知之甚少。我们推测，大鼠睾丸免疫激活后，炎症相关miRNA的表达会发生改变。
从接受5mg/kg腹腔注射细菌脂多糖（LPS）3或6小时后的Sprague-Dawley大鼠中纯化睾丸总RNA，并使用84个miRNA芯片通过qPCR进行检测。
在3小时组中，5种炎症相关miRNA表现出超过两倍的下调（P<0.05）（大鼠let-7f-5p、大鼠miR-200c-3p、大鼠miR-23a-3p、大鼠miR-23b-3p、大鼠miR-98-5p），6小时组中有5种（大鼠miR-17-5p、大鼠miR-19a-3p、大鼠miR-34a-5p、大鼠miR-34c-5p、大鼠miR-449a-5p）。
文献综述表明，这些miRNA在生育能力维持、癌症形成与消除以及雄性生殖道发育中也发挥着重要作用。进一步研究将有助于更深入地了解雄性生殖免疫学及相关健康问题。
