Zhu Yun-Chao, Zhan Guang-Jie, Yuan De-Pei, Yang Fu-Ming, Huang Qiong
School of Medical Science, HuBei University for Nationalities, Enshi 445000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Jan 8;33(1):51-55. doi: 10.12047/j.cjap.5448.2017.012.
To investigate the effects of Shiquanyuzhentang (Traditional Chinese Medicine) on the immunologic function of tu-mor-bearing mice and its mechanism of antitumor.
Thirty SPF grade male Kunming mice transplanted with H hepatocellular carci-noma were divided into three groups randomly:model group,positive control group and Shiquanyuzhentang group(=10). Another 10 mice were selected as normal control group. Normal control group and model group received normal saline and distilled water supplementation by 10 mL/kg everyday. Positive control group and Shiquanyuzhentang group received Shengyi(80 mg/ml)and Shiquanyuzhentang decoction at the doses of 8 g/kg and 18 g/kg respectively everyday. After 14 days of continuous administration, the mice were killed and the thymus, spleen index, tumor inhibition rate,peripheral blood leukocytes,lymphocyte content,cell percentage of T cell subsets CD3, CD4, CD8,interleukin 2 (IL-2)in serum,tumor necrosis factor-α(TNF-α),interferon β(IFN-β) content,lymphocyte proliferation ability and NK cell were measured.
Compared with normal control group, the weight of mice and thymus and spleen index of the Shiquanyuzhentang group were increased significantly(<0.05);leukocyte and lymphocyte CD3、CD4、CD8 and TNF-α were increased greatly(<0. 05),IL-2 and IFN-β were de-creased significantly(<0.05). Compared with model group,thymus and spleen index of Shiquanyuzhentang group were increased significant-ly(<0.05);leukocyte and lymphocyte CD3、CD4、IL-2、IFN-β and TNF-α of Shiquanyuzhentang group were increased significantly(<0. 05),CD8 content was decreased significantly(<0.05);NK cell and lymphocyte proliferation were increased significantly(<0.05).
Shiquanyuzhentang can promote the growth of immune organs in mice bearing H, enhance immune function and is beneficial to the recovery of tumor body.
探讨十全育真汤对荷瘤小鼠免疫功能的影响及其抗肿瘤作用机制。
将30只接种H肝癌的SPF级雄性昆明小鼠随机分为三组：模型组、阳性对照组和十全育真汤组（每组10只）。另选10只小鼠作为正常对照组。正常对照组和模型组每天按10 mL/kg补充生理盐水和蒸馏水。阳性对照组和十全育真汤组每天分别给予生益（80 mg/ml）和十全育真汤水煎剂，剂量分别为8 g/kg和18 g/kg。连续给药14天后，处死小鼠，测定胸腺、脾脏指数、肿瘤抑制率、外周血白细胞、淋巴细胞含量、T细胞亚群CD3、CD4、CD8的细胞百分比、血清白细胞介素2（IL-2）、肿瘤坏死因子-α（TNF-α）、干扰素β（IFN-β）含量、淋巴细胞增殖能力和NK细胞。
与正常对照组相比，十全育真汤组小鼠体重及胸腺和脾脏指数显著增加（P<0.05）；白细胞及淋巴细胞CD3、CD4、CD8和TNF-α显著升高（P<0.05），IL-2和IFN-β显著降低（P<0.05）。与模型组相比，十全育真汤组胸腺和脾脏指数显著增加（P<0.05）；十全育真汤组白细胞及淋巴细胞CD3、CD4、IL-2、IFN-β和TNF-α显著升高（P<0.05），CD8含量显著降低（P<0.05）；NK细胞和淋巴细胞增殖显著增加（P<0.05）。
十全育真汤可促进荷H小鼠免疫器官生长，增强免疫功能，有利于瘤体恢复。
