脂联素后处理对大鼠心肌缺血/再灌注损伤的影响及ADP/PI3K/Akt信号通路在脂联素后处理中的作用

[Effect of adiponectin postconditioning against myocardial ischemia/reperfusion injury in rats and role of ADP/PI3K/Akt pathway in adiponectin postconditioning].

作者信息

Zhao Lin-Jing, Cui Liu-Su, Zhang Jin-Ying, Wang Yong-Ling

机构信息

School of Basic Medicine, Xinxiang Medical University, Xinxiang 453003.

Department of Vasculocardiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450000, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Apr 8;33(4):308-313. doi: 10.12047/j.cjap.5531.2017.075.

DOI:10.12047/j.cjap.5531.2017.075

PMID:29926635

Abstract

OBJECTIVE

To investigate the effects of adiponectin(ADP) postconditioning against myocardial ischemia/reperfusion injury(MIRI) in rats and role of ADP/PI3K/Akt pathway in ADP postconditioning.

METHODS

SD rat was connected to ventilator by tracheal intubation under anesthesia, then left anterior descending coronary artery (LAD) was threaded between left auricle and pulmonary artery cone after exposing heart by surgery. MIRI model was induced by ligation of LAD for 30 min and the following reperfusion for 120 min. Rats were divided randomly into 5 groups (=12):① Sham group:LAD was threaded without ligation; ② MIRI group; ③ADP group (ADP postconditioning) were subjected to intravenous injection of ADP when LAD ligation for 10 min and the ligation held for 20 min after that, then reperfusion for 120 min; ④ ADP+LY294002 group were subjected to injection of ADP and LY294002 when LAD ligation for 10 min, the other steps were the same as ADP group; ⑤ LY294002 group were subjected to injection of LY294002 when LAD ligation for 10 min, the other steps were the same as ADP group. Titers of lactate dehydrogenase(LDH) and cardiac troponin I(cTnI) in plasma were observed, expressions of PI3K, Akt, phosphorylated-Akt(p-Akt), ADP mRNA, ADPR1 mRNA and PI3k mRNA in myocardial tissue were measured.

RESULTS

Compared with sham group, the levels of LDH and cTnI in MIRI group were increased (<0.05); Compared with MIRI group, the levels of LDH and cTnI in ADP group were decreased (<0.05); Compared with ADP group, the levels of LDH and cTnI were increased in LY294002 applying groups(<0.05). Compared with MIRI group, the expressions of PI3K, p-Akt, ADP mRNA, ADPR1 mRNA and PI3K mRNA were increased in ADP group (<0.05), the above mentioned 5 parameters in LY294002 applying groups were decreased(<0.05).

CONCLUSIONS

ADP postconditioning could reduce MIRI in rats, the protective effect might have relation to ADP/PI3k/Akt pathway.

摘要

目的

探讨脂联素（ADP）后处理对大鼠心肌缺血/再灌注损伤（MIRI）的影响及 ADP/PI3K/Akt 信号通路在 ADP 后处理中的作用。

方法

将 SD 大鼠麻醉后气管插管连接呼吸机，手术暴露心脏后，在左心耳与肺动脉圆锥之间穿线结扎左冠状动脉前降支（LAD）。结扎 LAD 30 min 后再灌注 120 min 建立 MIRI 模型。将大鼠随机分为 5 组（每组 12 只）：①假手术组：穿线但不结扎 LAD；②MIRI 组；③ADP 组（ADP 后处理）：LAD 结扎 10 min 时静脉注射 ADP，持续结扎 20 min 后再灌注 120 min；④ADP+LY294002 组：LAD 结扎 10 min 时注射 ADP 和 LY294002，其余步骤同 ADP 组；⑤LY294002 组：LAD 结扎 10 min 时注射 LY294002，其余步骤同 ADP 组。观察血浆中乳酸脱氢酶（LDH）和心肌肌钙蛋白 I（cTnI）水平，检测心肌组织中 PI3K、Akt、磷酸化 Akt（p-Akt）、ADP mRNA、ADPR1 mRNA 和 PI3k mRNA 的表达。

结果

与假手术组比较，MIRI 组 LDH 和 cTnI 水平升高（P<0.05）；与 MIRI 组比较，ADP 组 LDH 和 cTnI 水平降低（P<0.05）；与 ADP 组比较，应用 LY294002 组 LDH 和 cTnI 水平升高（P<0.05）。与 MIRI 组比较，ADP 组 PI3K、p-Akt、ADP mRNA、ADPR1 mRNA 和 PI3K mRNA 表达升高（P<0.05），应用 LY294002 组上述 5 项指标降低（P<0.05）。