Zhu Ze-Mei, Yang Ji-Hua, Yang Si-Si, He Jian-Qin, Zhang Kai-Fang, Feng Shui-Dong, Ling Hong-Yan
Department of Medicine, Changde Vocational Technical College, Changde 415003.
Department of Physiology, University of South China, University of South China, Hengyang 421001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2017 Apr 8;33(4):323-328. doi: 10.12047/j.cjap.5478.2017.079.
To observe the effects of dihydromyricetin(DHM) on cognitive dysfunction and expression of brain derived neurotrophic factor(BDNF) protein in hippocampus of type 2 diabetic mice(T2DM).
Forty C57BL/6J mice were randomly divided into two groups, normal control group (=8):normal diet feeding; T2DM model group (=32):high-glucose and high-fat combined with 100 mg/kg streptozocin(STZ) treatment (five mice died during modeling and three failed). Twenty-four diabetic mice were modeled successfully and divided into three groups (T2DM group, T2DM+L-DHM group and T2DM+H-DHM group). Three groups mice were fed with high-glucose and high-fat diet, and treated with equal volume of normal saline, 125 mg/(kg·d) DHM or 250 mg/(kg·d) DHM for 16 weeks respectively. The control mice were fed with normal diet and treated with equal volume of saline (once a day, gavage) for 16 weeks. After 16 weeks, the body weight and fasting blood glucose were measured, intraperitoneal glucose tolerance test and related behavioral experiment were performed. Finally, the expression of BDNF protein in hippocampus of mice was detected by Western blot.
The model of type 2 diabetes mellitus was established successfully with high-glucose and high-fat combined with 100 mg/kg STZ. After 16 weeks, the body weight of T2DM group was significantly decreased, the fasting blood glucose was significantly increased and the glucose tolerance was significantly abnormal compared with the normal control group. Compared with T2DM group, the body weight of T2DM+DHM groups mice was increased, while the levels of fasting blood glucose were decreased. And H-DHM could significantly improve the abnormal glucose tolerance of T2DM mice. Behavior test results showed that the ability of learning and memory of T2DM mice was significant decreased compared with control group, but these phenomena were improved in T2DM+DHM groups mice, and T2DM+H-DHM group was more obvious. Western blot analysis showed that the expression of BDNF protein in hippocampus of T2DM group was significantly lower than that of control group, while T2DM+DHM group was significant increased compared with T2DM mice.
Dihydromyricetin can improve the cognitive dysfunction in type 2 diabetic mice. The mechanism may be through hypoglycemic effect and activation of BDNF protein expression in hippocampus.
观察二氢杨梅素（DHM）对2型糖尿病小鼠（T2DM）认知功能障碍及海马脑源性神经营养因子（BDNF）蛋白表达的影响。
将40只C57BL/6J小鼠随机分为两组，正常对照组（n = 8）：正常饮食喂养；T2DM模型组（n = 32）：高糖高脂联合100 mg/kg链脲佐菌素（STZ）处理（建模过程中有5只小鼠死亡，3只建模失败）。24只糖尿病小鼠建模成功后分为三组（T2DM组、T2DM + L - DHM组和T2DM + H - DHM组）。三组小鼠均给予高糖高脂饮食，分别用等体积生理盐水、125 mg/(kg·d) DHM或250 mg/(kg·d) DHM处理16周。对照组小鼠给予正常饮食，并用等体积生理盐水（每日1次，灌胃）处理16周。16周后，测量体重和空腹血糖，进行腹腔葡萄糖耐量试验及相关行为学实验。最后，通过蛋白质免疫印迹法检测小鼠海马中BDNF蛋白的表达。
高糖高脂联合100 mg/kg STZ成功建立了2型糖尿病模型。16周后，与正常对照组相比，T2DM组小鼠体重显著下降，空腹血糖显著升高，葡萄糖耐量显著异常。与T2DM组相比，T2DM + DHM组小鼠体重增加，空腹血糖水平降低。且H - DHM能显著改善T2DM小鼠异常的葡萄糖耐量。行为学测试结果显示，与对照组相比，T2DM组小鼠学习记忆能力显著下降，但在T2DM + DHM组小鼠中这些现象得到改善，且T2DM + H - DHM组更明显。蛋白质免疫印迹分析显示，T2DM组小鼠海马中BDNF蛋白表达显著低于对照组，而T2DM + DHM组与T2DM小鼠相比显著增加。
二氢杨梅素可改善2型糖尿病小鼠的认知功能障碍。其机制可能是通过降血糖作用及激活海马中BDNF蛋白的表达。
