二氢杨梅素对高脂饮食诱导的小鼠肥胖的影响及其机制

[Effects of dihydromyricetin on high fat diet induced obesity in mice and its mechanism].

作者信息

Luo Jin-Ding, Wu Di, Lyu Hui-Jie, He Jian-Qin, Yang Si-Si, Feng Shui-Dong, Ling Hong-Yan

机构信息

Department of Physiology, University of South China.

Department of Social Medicine and Health Management, University of South China, Hengyang 421001, China.

出版信息

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2020 Jan 28;36(1):6-11. doi: 10.12047/j.cjap.5851.2020.002.

DOI:10.12047/j.cjap.5851.2020.002

PMID:32476366

Abstract

OBJECTIVE

To observe the effects of dihydromyricetin (DHM) on obesity induced by high-fat diet in mice, and to explore whether its mechanism of action is related to the promotion of WAT browning.

METHODS

Sixty c57bl/6j mice were randomly divided into 6 groups (n=10): ①normal control group (ND group): normal feed feeding; ②Normal control + low dose DHM group (ND+L-DHM group): normal feed feeding was treated with low dose DHM (125 mg/(kg·d)); ③Normal control + high dose DHM group (ND+H-DHM group): normal feed feeding was treated with high dose DHM (250 mg/(kg·d)); ④High-fat diet group (HFD): high-fat diet; ⑤high-fat diet + low-dose DHM group (HFD+L-DHM group): high-fat diet feeding with low-dose DHM; ⑥High-fat diet + high-dose DHM group (HFD+H-DHM group): High-fat diet was treated with high-dose DHM. After 16 weeks, the mice were fasted overnight, blood samples were collected for fasting blood glucose and blood lipids, then the animals were sacrificed, body length was measured, and Lee's index was calculated. After weighing the adipose tissue in the scapula, groin and epididymis, formaldehyde fixation and HE staining were used to observe the fat cells size, immunohistochemistry was used to detect the expression of uncoupling protein 1 (UCP1). The body weight was measured every 4 weeks during the experiment.

RESULTS

Compared with the ND group, the body weight of the mice in the HFD group was increased significantly, suggesting that the obese mouse model replicated successfully. In addition, the body fat weight, fat cell diameter, Lee's index and blood glucose of the HFD group were increased significantly, and the expression of UCP1 in the adipocytes was increased. Body weight, fat cell diameter, Lee's index and blood glucose of HFD mice treated with L-DHM and H-DHM were reversed significantly, while the expression of UCP1 in adipocytes was more significantly increased; however, L-DHM and H-DHM had no significant effects on the above indicators in normal mice.

CONCLUSION

Dihydromyricetin inhibited high fat diet induced mouse obesity; the mechanism might be associated with promoting WAT browning.

摘要

目的

观察二氢杨梅素（DHM）对高脂饮食诱导的小鼠肥胖的影响，并探讨其作用机制是否与促进白色脂肪组织（WAT）褐变有关。

方法

将60只c57bl/6j小鼠随机分为6组（n = 10）：①正常对照组（ND组）：给予正常饲料喂养；②正常对照+低剂量DHM组（ND+L-DHM组）：正常饲料喂养并给予低剂量DHM（125 mg/(kg·d)）；③正常对照+高剂量DHM组（ND+H-DHM组）：正常饲料喂养并给予高剂量DHM（250 mg/(kg·d)）；④高脂饮食组（HFD）：给予高脂饮食；⑤高脂饮食+低剂量DHM组（HFD+L-DHM组）：高脂饮食喂养并给予低剂量DHM；⑥高脂饮食+高剂量DHM组（HFD+H-DHM组）：高脂饮食喂养并给予高剂量DHM。16周后，小鼠隔夜禁食，采集血样检测空腹血糖和血脂，然后处死动物，测量体长并计算Lee's指数。称量肩胛、腹股沟和附睾处的脂肪组织重量后，用甲醛固定并进行苏木精-伊红（HE）染色观察脂肪细胞大小，采用免疫组化法检测解偶联蛋白1（UCP1）的表达。实验期间每4周测量一次体重。

结果

与ND组相比，HFD组小鼠体重显著增加，表明成功复制了肥胖小鼠模型。此外，HFD组的体脂重量、脂肪细胞直径、Lee's指数和血糖均显著增加，脂肪细胞中UCP1的表达增加。给予L-DHM和H-DHM处理的HFD小鼠的体重、脂肪细胞直径、Lee's指数和血糖均显著逆转，而脂肪细胞中UCP1的表达增加更为显著；然而，L-DHM和H-DHM对正常小鼠的上述指标无显著影响。