Wang Ling-Xing, Huang Hong-Hong, Lv Guo-Rong
Department of Neurology, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
Department of Ultrasound, the Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Jan 8;34(1):35-38. doi: 10.12047/j.cjap.5592.2018.010.
To evaluate the effect of prenatal mobile phone exposure on the expression of proliferating cell nuclear antigen (PCNA) and doublecortin (DCX) in dentate gyrus of offspring rats.
The rat model of prenatal mobile phone exposure was established and there were three groups including control group, short term maternal exposure group and long term maternal exposure group(=6). From pregnant day 1 to day 17, pregnant rats in long term and short term maternal exposure group were exposed to an mobile phone in talking mode for 6 h/d and 24 h/d, respectively. Length of pregnancy, maternal body weight gain, litter size and pup's body weight were observed. The cell morphology in dentate gyrus of offspring rats at the age of 1 month was studied by cresyl violet staining. The immunohistochemical expression of PCNA and DCX in dentate gyrus of rat offspring were detected, and the expression of DCX and brain derived neurotrophic factor (BDNF) in hippocampus of rat offspring were evaluated by Western blot.
There was no difference in length of pregnancy, maternal body weight gain, litter size and pup's body weight among three groups. The morphological changes of pyramidal cells in the polymorphic layer and DCX-positive cells in the dentate gyrus were obvious in rat offspring of long term maternal exposure group. There were less PCNA-positive cells in dentate gyrus and decreased expression of DCX and BDNF in hippocampus by Western blot in long term maternal exposure group compared with control and short term maternal exposure group (all <0.05).
Long term prenatal mobile phone exposure might inhibit the expression of PCNA and DCX in dentate gyrus of rat offspring by down-regulating BDNF.
评估孕期手机暴露对仔鼠齿状回中增殖细胞核抗原（PCNA）和双皮质素（DCX）表达的影响。
建立孕期手机暴露大鼠模型，分为对照组、孕期短期暴露组和孕期长期暴露组（每组n = 6）。从妊娠第1天至第17天，孕期长期暴露组和短期暴露组的孕鼠分别以通话模式每天暴露于手机6小时和24小时。观察孕期时长、母鼠体重增加量、窝仔数和仔鼠体重。采用甲酚紫染色研究1月龄仔鼠齿状回的细胞形态。检测仔鼠齿状回中PCNA和DCX的免疫组化表达，并通过蛋白质印迹法评估仔鼠海马中DCX和脑源性神经营养因子（BDNF）的表达。
三组之间的孕期时长、母鼠体重增加量、窝仔数和仔鼠体重均无差异。孕期长期暴露组仔鼠多形层锥体细胞和齿状回DCX阳性细胞的形态学变化明显。与对照组和孕期短期暴露组相比，孕期长期暴露组齿状回中PCNA阳性细胞较少，蛋白质印迹法显示其海马中DCX和BDNF的表达降低（均P < 0.05）。
孕期长期手机暴露可能通过下调BDNF抑制仔鼠齿状回中PCNA和DCX的表达。
