Hwang In Koo, Yoo Ki-Yeon, Li Hua, Choi Jung Hoon, Kwon Young-Guen, Ahn Youngkeun, Lee In Se, Won Moo Ho
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, South Korea.
Neurochem Res. 2007 Sep;32(9):1604-9. doi: 10.1007/s11064-007-9366-1. Epub 2007 May 19.
Doublecortin (DCX), a microtubule-associated protein, specifically expresses in neuronal precursors. This protein has been used as a marker for neuronal precursors and neurogenesis. In the present study, we observed differences in DCX immunoreactivity and its protein levels in the hippocampal dentate gyrus between adult and aged dogs. In the adult dog, DCX immunoreactive cells with well-stained processes were detected in the subgranular zone of the dentate gyrus. Numbers of DCX immunoreactive cells in the dentate gyrus of the aged dog were significantly decreased compared to those in the adult dog. DCX immunoreactive cells in both adult and aged dog did not show NeuN (a marker for mature neurons) immunoreactivity. NeuN immunoreactivity in the aged dog was poor compared to that in the adult dog. DCX protein level in the aged dentate gyrus was decreased by 80% compared to that in the adult dog. These results suggest that the reduction of DCX in the aged hippocampal dentate gyrus may be involved in some neural deficits related to the hippocampus.
双皮质素（DCX）是一种微管相关蛋白，在神经元前体细胞中特异性表达。该蛋白已被用作神经元前体细胞和神经发生的标志物。在本研究中，我们观察了成年犬和老年犬海马齿状回中DCX免疫反应性及其蛋白水平的差异。在成年犬中，在齿状回的颗粒下区检测到具有染色良好突起的DCX免疫反应性细胞。与成年犬相比，老年犬齿状回中DCX免疫反应性细胞的数量显著减少。成年犬和老年犬中的DCX免疫反应性细胞均未显示NeuN（成熟神经元的标志物）免疫反应性。与成年犬相比，老年犬中的NeuN免疫反应性较差。与成年犬相比，老年齿状回中的DCX蛋白水平降低了80%。这些结果表明，老年海马齿状回中DCX的减少可能与一些与海马体相关的神经缺陷有关。
