1 Nanoscience and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.
2 Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan.
Appl Spectrosc. 2018 Aug;72(8):1137-1169. doi: 10.1177/0003702818778851. Epub 2018 Jun 21.
The introduction of light sheet fluorescence microscopy (LSFM) has overcome the challenges in conventional optical microscopy. Among the recent breakthroughs in fluorescence microscopy, LSFM had been proven to provide a high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and minuscule levels of phototoxic and photodamage effects. The aforementioned auspicious properties are crucial in the biomedical and clinical research fields, covering a broad range of applications: from the super-resolution imaging of intracellular dynamics in a single cell to the high spatiotemporal resolution imaging of developmental dynamics in an entirely large organism. In this review, we provided a systematic outline of the historical development of LSFM, detailed discussion on the variants and improvements of LSFM, and delineation on the most recent technological advancements of LSFM and its potential applications in single molecule/particle detection, single-molecule super-resolution imaging, imaging intracellular dynamics of a single cell, multicellular imaging: cell-cell and cell-matrix interactions, plant developmental biology, and brain imaging and developmental biology.
光片荧光显微镜（LSFM）的引入克服了传统光学显微镜的挑战。在荧光显微镜的最新突破中，LSFM 已被证明能够提供高的三维空间分辨率、高的信噪比、快速的成像采集速度和微小的光毒性和光损伤效应。上述有利特性在生物医学和临床研究领域中至关重要，涵盖了广泛的应用：从单个细胞内动力学的超分辨率成像到整个大型生物体发育动力学的高时空分辨率成像。在这篇综述中，我们系统地概述了 LSFM 的历史发展，详细讨论了 LSFM 的变体和改进，并阐述了 LSFM 的最新技术进展及其在单分子/颗粒检测、单分子超分辨率成像、单个细胞内动力学成像、多细胞成像：细胞-细胞和细胞-基质相互作用、植物发育生物学以及脑成像和发育生物学中的潜在应用。
